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胰腺癌干细胞中受OCT4调控的表面标志研究 被引量：1

A Preliminary Study on the Surface Marker of Human Pancreatic Cancer Stem Cell Regulated by OCT4
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摘要 OCT4和Nanog被公认是人ESC的自我更新调控基因,其中OCT4能够转录调控多种表面蛋白的表达,如SEMA6A。该文将人胰腺癌细胞株Panc-1、Bxpc-3、Aspc-1和Cfpac-1培养在无血清条件下,采用EGF、IGF-1和FGF-10诱导球体形成。用免疫荧光法分别检测这4种人胰腺癌细胞株及其球体细胞以及15例胰腺癌组织标本和13例正常胰腺组织标本中OCT4和Nanog的表达,结果显示,4种人胰腺癌细胞株在无血清-DF12培养基中5～10 d即可形成悬浮生长的球体。OCT4和Nanog在4种细胞株均有表达,且球体细胞中表达明显高于亲代细胞。在胰腺癌组织中仅有少量表达自我更新基因,在正常胰腺组织中微量表达。此外,还检测到Panc-1球体细胞表面高表达SEMA6A。由此可见,自我更新基因OCT4和Nanog在胰腺癌细胞中的表达和CSC有关,其表面蛋白SEMA6A作为胰腺癌干细胞表面标志物值得进一步研究。 OCT4 and Nanog are two core transcriptional factors to regulate the self renewal in human embryonic stem cells （hECSs）. The expression of SEMA6A membrane protein in hESCs is regulated by OCT4. In this paper, we induced the sphere formation in Panc-1, Bxpc-3, Aspc-1 and Cfpac-1 pancreatic cancer cell lines by culturing the cells in the serum-free conditions supplemented with EGF, IGF-1 and FGF-10. Their expression of selfrenewing genes, OCT4 and Nanog, were measured by immunofluorscent staining. The same assay was also done in these cell lines including 15 cases of pancreatic cancer tissues and 13 cases of normal pancreas. The float-growing spheres were developed after 5 to 10 days culture in all the cell lines tested. The expression of OCT4 and Nanog in the sphere-forming cells was much higher than their relevant counterparts in cell lines. These stemness markers were also found in the pancreatic cancer tissues and at much lower level in normal pancreas. Furthermore, the stemlike spheres in Panc-1 were observed to express SEMA6A, a surface marker known to be the OCT4 downstream target. In conclusion, the expression of self-renewing genes, OCT4 and Nanog, in pancreatic cancer cells implies their relevance to the cancer stem cells. The SEMA6A protein regulated by OCT4 may represent an invaluable sur- face marker for studying the putative pancreatic cancer stem cells.
作者 周竹超 董强刚 倪泉兴
机构地区 复旦大学附属华山医院普外科 上海市肿瘤研究所分子诊断实验室 复旦大学附属肿瘤医院胰腺外科
出处 《中国细胞生物学学报》 CAS CSCD 2011年第6期629-634,共6页 Chinese Journal of Cell Biology
关键词 胰腺癌 肿瘤干细胞 自我更新 球体 分子标志物 pancreatic cancer cancer stem cells self-renewal sphere molecule marker
分类号 R735.9 [医药卫生—肿瘤]
  • 相关文献

参考文献16

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共引文献20

同被引文献26

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中国细胞生物学学报
2011年 第6期

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