PVY株系间的分子变异及分子鉴定方法

Molecular Variation and Identification of Potato Virus Y Strains

马铃薯Y病毒(Potato virus Y,PVY)株系分化现象明显,难于准确鉴定。本研究利用核酸序列分析工具对PVY主要株系的全基因组序列、cp基因序列和p1基因序列进行了系统分析,探明了PVY主要株系的分子特征。结果表明,具有PVYN株系血清型的PVYN、PVYNTN株系与具有PVYO株系血清型的PVYO、PVYN:O、PVYNW株系间在cp基因第50位和第86位核苷酸处存在特异性分子变异;将p1基因与cp基因联合进行序列分析可对PVY各主要株系进行准确的鉴定,并建立了具体的分析方法。

Strain differentiation of potato virus Y（PVY） is obvious,and it is difficult to be identified.The complete genome,cp and p1 genes of different PVY strains were analyzed with informatics tools,and the molecular variation characters among different PVY strains were proved up.It was confirmed that the nucleotide at 50 nt sites and 86 nt sites in cp gene was different among the strains which belong to PVYN serotype（PVYN,PVYNTN） and PVYO serotype（PVYO,PVYN ： O,and PVYNW）.It was confirmed that the PVY strains could be identified with the analyses of combined nucleotide sequence of p1 and cp genes,and the identification method was established.