P16、P15、Smad7及TGF-β1在宫颈癌中的表达及意义

Expressions and significances of P16,P15,Smad7 and TGF-β1 in cervical cancer

目的:研究P16、P15、Smad7和TGF-β1在宫颈癌中的表达,研究其在不同临床病理特征中的意义。方法:选取三峡大学医学院自2008年1月～2010年5月收集的76例宫颈癌标本作为实验组,以50例正常宫颈组织作为对照组,采用免疫组织化学技术,分别检测P16、P15、Smad7及TGF-β1在两组中的表达情况,以及在肿瘤不同临床病理特征之间的关系。结果:①P16及P15蛋白在宫颈癌组织中的表达率与正常宫颈组织中P16、P15的表达率比较,差异具有统计学意义(P<0.05);②P16、P15蛋白的阳性表达率与宫颈组织的病理学分级具有相关性,P16、P15在宫颈癌Ⅰ级中的阳性表达率明显高于Ⅱ级和Ⅲ级;③Smad7、TGF-β1在宫颈癌组织中表达的阳性率明显高于对照组(P<0.05),同时Smad7、TGF-β1的表达与宫颈癌的临床分期及淋巴结转移密切相关(P<0.05)。结论:P16、P15、Smad7、TGF-β1的表达与宫颈癌的发生和进展有一定的相关性,其表达的降低或缺失在宫颈癌的发展过程中具有协同作用,检验P16、P15、Smad7、TGF-β1的表达可以作为判定宫颈癌危险程度的指标,为正确的评估宫颈癌患者的预后提供依据。

Objective：To study the expressions of P16,P15,Smad7 and TGF-β1 in cervical cancer,research their significances in different clinicopathologic features.Methods：76 specimens of cervical cancer were collected from the hospital as experimental group from January 2008 to May 2010,50 cases with normal cervical tissues were selected as control group,immunohistochemical technique was used to detect the expression levels of P16,P15,Smad7 and TGF-β1 in the two groups and their relationships with different clinicopathologic features.Results：There was significant difference in expression rates of P16 protein and PI5 protein between experimental group and control group（P0.05）;there was correlation between positive expression rates of P16 protein and PI5 protein and pathological grades of cervical tissues,the positive expression rates of P16 protein and PI5 protein in cervical cancer of grade Ⅰ were significantly higher than those in cervical cancer of grade Ⅱ and Ⅲ;the positive expression rates of Smad7 and TGF-β1 in experimental group were significantly higher than those in control group（P0.05）,the expression rates of Smad7 and TGF-β1 were closely related to clinical stages of cervical cancer and lymph node metastasis（P0.05）.Conclusion：There was a certain correlation between expressions of P16,P15,Smad7,TGF-β1 and the occurrence and development of cervical cancer,the down-regulation and deletion of their expressions play synergistic effects in the course of cervical cancer development,detections of P16,P15,Smad7 and TGF-β1 can be used as indexes to determine the degree of risk of cervical cancer,and provide a basis for evaluating the prognosis of patients with cervical cancer correctly.