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生精胶囊促进小鼠精原干细胞增殖的研究

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摘要 为了探讨体外促进小鼠精原干细胞(SSCs)增殖的可行性,试验取7~8日龄雄性乳鼠睾丸,分离、纯化SSCs并分别接种于普通培养液、神经营养因子(GDNF)培养液和生精胶囊提取液的培养液内进行增殖培养,观察小鼠SSCs的生长状况。结果表明:将SSCs接种于普通培养液3 d后,出现了增殖现象,可见增殖培养产生SSCs克隆,并可连续传2代;生精胶囊提取液组和GDNF组于2 d后出现SSCs增殖并产生克隆,且传至5代。说明生精胶囊提取液可以在体外促进小鼠SSCs的增殖。
出处 《黑龙江畜牧兽医》 CAS 北大核心 2011年第6期51-53,共3页 Heilongjiang Animal Science And veterinary Medicine
基金 辽宁省教育厅高等学校科学研究项目(L2010263) 辽宁医学院博士科研启动基金项目(2008D11)
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