电离辐射诱导人外周血线粒体DNA4977 bp缺失的初步定量PCR分析

Primary Quantitative Analysis of the mtDNA4977bp Deletion Induced by Ionizing Radiation in Human Peripheral Blood Using real-time PCR

目的观察不同剂量γ射线对人外周血线粒体DNA4977bp缺失的影响,探索线粒体DNA4977bp缺失作为生物剂量计的可行性。方法采集3名健康人外周血进行离体照射,利用荧光定量PCR技术,检测射线照射后外周血细胞中线粒体DNA4977bp缺失的发生情况。结果发现0-8Gy范围内,随着剂量的增加mtDNA49bp相对缺失量有增加的趋势,使用Origin7.5统计软件进行拟合,建立了以人血细胞mtDNA4977bp缺失为指标的剂量效应关系曲线方程Y=1.2693＋1.0660X＋0.0198X2。结论不同剂量γ射线对人外周血线粒体DNA4977bp缺失的有一定的影响,且线粒体DNA4977bp与受照剂量有一定的效应关系,因此,线粒体DNA4977bp缺失在生物剂量估算中具有潜在的应用价值。

Objective To observe the influence of mtDNA4977bp deletion induced by different dose of γ ray in human peripheral blood in order to explore the feasibility of mtDNA4977bp deletion as biodosimeter.Methods Human peripheral blood samples were collected from three healthy donors and irradiated by γ ray,MtDNA4977bp deletion was detected by real-time PCR.Results It indicated that that from the range of 0~8 Gy,the relationship between mtDNA4977bp deletion and irradiation dose represents certain curvilinear correlation（Y=1.2693＋1.0660X＋0.0198X2）.Conclusion We find that γ ray has influence on the mtDNA4977bp deletion,so it may be an important biodosmeter in future.