摘要
采用显色平板法和测定脱乙酰度(Deacetylation degree,DD)相结合的方法,从浅海污泥中筛选到一株产几丁质脱乙酰酶(chitin deacetylase,简称CDA)活力较高的细菌菌株S-1,并对其产酶条件和发酵进程进行了初步研究。S-1适宜产酶条件:碳源为蔗糖,氮源为酵母膏,添加无机盐NaH2PO4,培养时间54h。
A bacterium S-1 which produces high content of chitin deacetylase was screened from neritic mud with the method of color reaction plate and determining the degree of deacetylation. The optimal production conditions of chitin deacetylation by this strain were further studied and obtained as followed: carbon source sucrose, nitrogen source yeast extract and the culture time 54h in the presence of NaH2PO4.
出处
《中国酿造》
CAS
北大核心
2011年第6期61-64,共4页
China Brewing
基金
山东省自然科学基金(Y2008D26)