混菌固态发酵麸皮制备蛋白饲料的研究

Production of protein feed from wheat bran with solid-state fermentation by multi-strains

从4株酵母菌中筛选出一株在麸皮培养基中合成蛋白能力较高的酵母菌Ⅱ,然后,利用酵母菌Ⅱ与高产纤维素酶活菌株黑曲霉Ⅰ之间的协同效应,以麸皮为底物,混菌固态发酵生产蛋白饲料。通过单因素和正交试验优化发酵条件。结果表明,培养温度30℃、接菌量10%、接菌比酵母菌Ⅱ∶黑曲霉Ⅰ(2∶1)、水料比(1∶1)、葡萄糖添加量2%,发酵产物中粗蛋白含量为25.26%,比麸皮发酵前粗蛋白含量提高了33.93%。

Yeast II with high capacity of protein synthesis in the wheat bran medium was selected from 4 strains of yeast. Yeast II and the stain of Aspergillus niger I with high cellulase activity were used for protein feed production with solid-state fermentation of multi-strains. Optimal fermentation conditions were obtained with single factor experiment and orthogonal experiment as followed： fermentation temperature 30~C, inoculum 10%, the inoculation ratio of yeast II and A. niger I 2：1, the liquid-solid ratio 1：1 and the glucose 2%. Under these conditions, the content of crude protein in fermentation product reached 25.26%, which was increased by 33.93% compared to that before fermentation.