摘要
从4株酵母菌中筛选出一株在麸皮培养基中合成蛋白能力较高的酵母菌Ⅱ,然后,利用酵母菌Ⅱ与高产纤维素酶活菌株黑曲霉Ⅰ之间的协同效应,以麸皮为底物,混菌固态发酵生产蛋白饲料。通过单因素和正交试验优化发酵条件。结果表明,培养温度30℃、接菌量10%、接菌比酵母菌Ⅱ∶黑曲霉Ⅰ(2∶1)、水料比(1∶1)、葡萄糖添加量2%,发酵产物中粗蛋白含量为25.26%,比麸皮发酵前粗蛋白含量提高了33.93%。
Yeast II with high capacity of protein synthesis in the wheat bran medium was selected from 4 strains of yeast. Yeast II and the stain of Aspergillus niger I with high cellulase activity were used for protein feed production with solid-state fermentation of multi-strains. Optimal fermentation conditions were obtained with single factor experiment and orthogonal experiment as followed: fermentation temperature 30~C, inoculum 10%, the inoculation ratio of yeast II and A. niger I 2:1, the liquid-solid ratio 1:1 and the glucose 2%. Under these conditions, the content of crude protein in fermentation product reached 25.26%, which was increased by 33.93% compared to that before fermentation.
出处
《中国酿造》
CAS
北大核心
2011年第6期74-77,共4页
China Brewing
基金
国家农转资金(2010GB2B000092)
国家星火计划(2010GA651004)
辽宁省科技攻关计划(2008301029)
关键词
麸皮
固态发酵
蛋白饲料
wheat bran
solid-state fermentation
protein feed