响应面法优化超临界CO_2萃取平贝总碱工艺的研究

Optimization of supercritical CO_2 extraction of total alkaloids from Fritillaria ussuriensis maxim using response surface methodology

利用超临界二氧化碳萃取技术提取平贝总碱,运用响应面法优化萃取工艺条件。在单因素试验基础上,以萃取压力、萃取温度、夹带剂用量为响应因素,平贝总碱萃取率为响应值,根据中心组合设计试验设计原理采用3因素3水平的响应面分析法,确定各工艺条件对萃取率的影响。结果表明:超临界二氧化碳萃取平贝总碱的最佳工艺条件为萃取压力22MPa、萃取温度48℃、夹带剂用量323mL。该条件下,萃取率为94.55%。

The supercritical carbon dioxide extraction technology of total alkaloids in Fritillaria ussuriensis maxim was optimized by response surface methodology combined with a 3-variable, 3-level central composite design（CCD）on the basis of single factor experiments. The extraction pressure, temperature and entrainer amount were response factors, and the extraction rate of total alkaloids in F. ussuriensis maxim was response value. The ef- fect of extraction conditions on the extraction rate was investigated. The results showed that the optimum extraction conditions were as follows： extraction pressure 22MPa, extraction temperature 48℃, entrainer amount 323ml. The extraction rate was 94.55% under optimum conditions.