摘要
文中对离子交换层析法分离RNA水解液中4种单核苷酸的工艺条件进行了研究。考察了上样pH值、上样量、洗脱剂、洗脱流速等因素对4种核苷酸分离效果的影响。研究确定的最佳分离条件为:核苷酸溶液pH值为2.0,上样量为1.0g/L树脂,洗脱液为蒸馏水,洗脱流速为0.15BV/h。经过2次上柱洗脱可将4种单核苷酸较好分离。
The factors on separation of four kinds of nucleotides from RNA hydrolysates by ion-exchange chromatography, such as sample pH value, sample quantity, eluant, eluting velocity were studied. The optimum technological conditions were determined as follows： sample pH value was 2.0, sample quantity was 1.0g/L ,resin, eluant was distilled water, eluting velocity was 0.15 times as the volume of column per hour. Four kinds of nucleotides were at last separated by two-step column chromatography.
出处
《中国酿造》
CAS
北大核心
2011年第6期143-147,共5页
China Brewing
关键词
核糖核酸
单核苷酸
离子交换柱层析
分离
ribonucleic acid
mononucleotide
ion exchange chromatography
separation
