摘要
通过单因子试验和正交试验对选育的一株产壳聚糖酶菌株UC-233进行发酵条件优化,结果表明其最适氮源是黄豆粕粉,最适碳源是壳聚糖,其最适发酵条件是:黄豆粕粉3.3%,胶体壳聚糖1.5%,培养基初始pH为4.0,250mL三角瓶装液量为50mL,接种量为1mL,发酵温度是30℃,发酵时间为48h,在此条件下菌株发酵液的壳聚糖酶活力达39.5U/mL,是优化前的1.43倍。
Optimization ofUC-233 fermentation conditions was studied via single factor and orthogonal test design. The results showed that： the optimum nitrogen source was soy meal powder, the optimum carbon source was colloid chitosan, the optimized fermentation conditions for chitosanase production were： soy meal powder 3.3%, colloid chitosan 1.5%, initial pH value 4.0,250ml flask containing 50ml medium, inoculation quantity lml, temperatm＇e was 35℃, fermentation time was 48h,Under these conditions, the highest chitosanase activity could reach 39.5U/ml, which was 1.43 times compared with that before optimization.
出处
《中国酿造》
CAS
北大核心
2011年第6期148-152,共5页
China Brewing
关键词
壳聚糖酶
发酵条件
优化
chitosanase
fermentation condition
optimization
