肝移植术后ImmuKnow细胞免疫功能与白细胞及T淋巴细胞计数的相关性研究

The relationship between ImmuKnow value of cellular immune function and peripheral white blood cells count and T.lymphocyte after liver transplantation

目的探讨肝移植术后ImmuKnow细胞免疫功能测定值与白细胞分类计数和T淋巴细胞亚群计数的相关性，为临床提供一种价格低廉快速判断肝移植受者细胞免疫功能的方法。方法选择49例行经典原位肝移植术受者术后2周至2个月内在无糖皮质激素应用情况下的外周血样本。分析ImmuKnow测定值与白细胞分类计数和T淋巴细胞亚群计数的相关性。并随机选择5例无激素免疫抑制的移植受者于术后2,3、4、6-,8周分别重复检测上述指标，进一步验证其相关性。结果白细胞总数与ImmuKnow ATP值相关性最高，相关系数为0．821；中性粒细胞计数与ImmuKnowATP值相关性次之，相关系数为0．787；单核细胞计数相关系数虽然有统计学意义，但相关系数低于0．5。淋巴细胞计数和淋巴细胞亚群计数与ImmuKnow ATP值的相关性无统计学意义。5例无激素免疫抑制受者术后重复检测ImmuKnow ATP值的变化与细胞总数的变化呈正相关，相关系数均〉0．5。结论肝移植术后早期白细胞计数与CD4＋T细胞ImmuKnow ATP值具有一定的正相关性，白细胞计数的变化，可以在一定程度上反映ImmuKnow ATP值的变化。

Objective To explore the relationship between peripheral differential blood count and ATP value in Cell CD4 ＋ T tested by ImmuKnow method in liver transplants. Methods In this study 49 recipients after classic orthotopic liver transplantation （OLT） were enrolled. In a period from two weeks to two months after transplantation when all were free of glucocorticoid. Blood were sent for WBC differential samples count and ATP value in Cell CD4 ＋ T tested by ImmuKnow method via SPSS17.0 software. Five more samples were selected randomly for duplicated testing of the indices in Week2, 3,4,6 and 8 after the transplanting operation to further verify the relativity. Results White blood cell count has the highest relativity with ImmuKnow ATP value at 0. 821. The 5 recipients were repeatedly tested for ImmuKnow ATP values that were found positively correlated to cell count with a coefficient of over 0. 5. Conclusions The peripheral leukocyte count in early stage after liver transplantation is in positive correlation with ATP value in Cell CD4 ＋ T, and the changes of numeration of leukocyte reflect changes of ATP value.