三倍体葡萄种质创新及倍性快速鉴定

Germplas Development of Triploid Grapevine and Rapid Identification of Chromosomes Ploidy

以二倍体葡萄品种玫瑰香、红地球、摩尔多瓦、无核奥迪亚及四倍体葡萄品种巨峰为亲本进行杂交授粉,对杂种进行胚培养,成株率最高可达30.6%。对杂种后代用流式细胞仪初选,确定30个杂交后代为三倍体植株。组合不同,获得三倍体植株的比例不同,目前获得的225个胚培养的葡萄杂种后代全部栽入大田。

Diploid grape＇Muscat Hamburg＇,＇Red Globe＇,＇Moldova＇and＇Otilia seedless＇were crossed with tetraploid parents＇Kyoho＇.The hybrid embryos were in vitro cultured and regenerated into plantlets.The regeneration rate of plantlets reaches to 30.6%.By using Flow Cytometry,thirty triploid cross progenies were obtained.The results showed that the percentages of triploid cross progenies was different when with the different cross combinations of parents.Recently 225 regenerated grape progenies were transplanted into field.