摘要
运用RT-PCR技术从由植物凝集素(PHA)诱导培养的牛外周血淋巴细胞(PBMC)扩增出牛白细胞介素6(IL6)完整开放阅读框(ORF),并通过序列比对、同源建模等方法对牛、人和大鼠的IL-6基因序列、可能的活性氨基酸残基、结构进行了比较分析,推测牛的IL-6基因可以在大鼠体内发挥较好的活性作用。
The interleukin-6 gene was cloned from peripheral blood mononuclear cell of bovine induced by phytohemagglutinin （PHA）. Comparable analysis for sequence, critical residues and structure of IL-6 were performed among bovine, human and rat using homology modeling and sequence alignment etc. The results showed that the bovine IL-6 may have a well reactivity on rat.
出处
《生物学杂志》
CAS
CSCD
2011年第3期1-4,共4页
Journal of Biology
基金
欧盟项目FMD-DISCONVAC(GrantNo.226556)
家畜疫病病原生物学国家重点实验室自主研究课题(No.SKLVEB2008ZZKT008)
甘肃省自然科学基金(GrantNo.0710RJZA082)
关键词
IL6
种属特异性
克隆
IL-6
species cross-reactivity
clone
