阴沟肠杆菌菌株中携带小肠结肠炎耶尔森菌毒力岛irp-2基因的检测

The pathogenicity island gene irp-2 of Yersinia enterocolitica was detected in Enterobacter gergoviae isolated in Yunnan China

目的:了解从云南战区感染性腹泻患者粪便标本中分离的阴沟肠杆菌是否携带小肠结肠炎耶尔森菌H IP(H ighPathogen ic ity Island)毒力岛irp-2基因,并研究菌株的毒力及耐药情况。方法:用PCR扩增法检测毒力岛irp-2基因,小白鼠腹腔注射检测毒力。结果:从1040例腹泻病人粪便分离的25株阴沟肠杆菌中,检测出18株携带小肠结肠炎耶尔森菌H IP毒力岛irp-2基因,毒力试验证实有较强毒力,药敏试验筛选出了敏感、中度敏感及耐药三类药敏谱。结论:云南战区检出的阴沟肠杆菌含有小肠结肠炎耶尔森菌H IP毒力岛irp-2基因且为毒力较强的菌株,对人类的健康具有潜在性的威胁。建立的药敏谱,为军队和地方今后在治疗由此菌引起的感染提供重要参考。

Objective：To investigate whether the enterobacter cloacaes separated from excrementitious samples of diarrhea Sufferers in Yunnan area was or not containing irp-2 gene of pathogenicity island of Yersinia enterocolitica,and then research virulence and resistance to drugs.Methods： The irp-2 gene of pathogenicity island is amplified by PCR,The virulence was detected by peritoneal injection of mouse.Results： The 18 among 25 strains separated from excrementitious samples of 1040 diarrhea patients was detected as carrying irp-2 gene of pathogenicity island of Yersinia enterocolitica by PCR.The virulence of strains were identified as velogen strain.The antibiotic sensitivity spectrum of susceptibility,medium sensitivity and drug fast were determined by drug sensitive test.Conclusion： The enterobacter cloacaes separated from Yunnan area containing irp-2 gene of pathogenicity island of Yersinia enterocolitica and were velogen strain.