L-半胱氨酸-原子荧光法测定食品中的硒

Determination of selenium in food by L-Cysteine atomic fluorescence spectrometry

目的:利用氢化物发生-原子荧光光谱法,在L-半胱氨酸存在下,测定食品中的硒。方法:样品经酸加热消化后,用2%盐酸溶液做载流,原子荧光分光光度计进行测定。结果:方法的测定范围为10.0μg/L^50.0μg/L,相对标准偏差为0.70%~1.90%,回收率为93.0%~101.0%,检出限为0.051μg/L。结论:由于L-半胱氨酸的存在,改善了氢化物发生条件,增敏光谱测定信号,降低了溶液的酸度,金属离子的干扰显著地得到了抑制,适用于食品中硒的测定。

Objective：In this paper,we give a method for determination of selenium in food by hydride generation-atomic fluorescence spectrometry in the presence of L-Cysteine.Methods： Using 2% hydrochloric acid as carrier liquid,the samples were determined by atomic fluorescence spectrometry after been digested by heating in acid.Results： The measurement range of this method was 10.0 μg/L^50.0 μg/L,the RSD 0.7%~1.9%,the recoveries were in the range of 93.0%~101.0% and the detection limits of this method were found to be 0.051 μg/L.Conclusion： In the presence of L-Cysteine,the hydride generation conditions were improved,the spectrometric signal was enhanced,and the acidity of solution was reduced.In addition,the interferences of the metal elements were significantly inhibited.The method can be used to determine seleium in food.