1342例流感样病例病毒核酸监测结果分析

Analysis of nucleic acid of influenza virus isolated from 1342 suspected influenza patients

目的：了解曲靖市流感病毒优势株的亚型变化和流感的流行趋势,为制定流感的防控策略和措施提供科学依据。方法：提取咽拭子标本中的病毒核酸,采用real tim e RT-PCR法进行核酸检测和型别鉴定。结果：2009年10月-2010年9月共采集流感样病例咽拭子1342份,检出流感病毒核酸阳性898份,阳性率66.92%。其中,甲（A）H1N1亚型564份,占62.81%;,甲（A）季3亚型19份,占2.12%;甲（A）季1亚型1份,占0.11%;B型159份,占17.71%;甲型未分型155份,占17.26%。男性阳性率为65.20%,女性阳性率为69.10%。结论：2009年10月-2010年2月,曲靖市流感疫情处于流感流行的高峰期,甲（A）H1N1流感病毒为流感流行主要毒株,2010年5月-2010年7月B型流感上升为主要流行毒株,甲（A）H1N1亚型、甲（A）季3亚型、B型流感病毒在整个监测期间交替出现,甲（A）H1N1亚型作为新出现的流感病毒型别,流行速度快,活动力强。15~岁年龄组人群是此流行期的高危人群,加强学校的流感监测与报告,落实相应的防控措施,将有助于流感的控制。

Objective：To determine the epidemic trends of influenza so as to provide scientific evidence to control it.Methods： Viral nucleic acid were extracted from nasopharyngeal swab.Real time RT-PCR was adopted to detect DNA and influenza type.Results： 1342 nasopharyngeal swab specimens were collected from Oct.2009 to Sep.2010.The positive rate of nucleic acid of influenza virus was 66.92%（898/1342）,of which 564（62.81%） were（A） H1N1 virus,19（2.12%） were（A）H3,1（0.11%） was（A） H1,159（17.71%） were B subtype virus.155（17.26%） was A subtypes unidentified.The positive rate in male were 65.2% and 69.10% in female.Conclusion： Oct.2009 to Feb.2010 was the influenza season in Qujing.（A）H1N1 was the dominant strains that cause the epidemic of influenza and B virus emerged from May to July 2010.（A）H1N1 H3N2 and B were detected alternately.As a new viral type,（A）H H1N1 is active and can spread quickly.Children under 15 years old are high risk group.The surveillance and reports in schools should be strengthened and positive measures should be taken to restrain the epidemic trends.