黔东南州2010年手足口病病原学监测结果

Monitoring result of Hand-foot-mouth disease pathogens in Qiandongnan Prefecture,Guizhou in 2010

目的:了解黔东南州2010年手足口病流行的主要病原体。方法:采集临床诊断为手足口病病例的咽拭子、粪便或肛拭子、疱疹液采用RT-PCR方法扩增病毒的特异性片段,检测EV71、CA16核酸和非EV71、CA16的其他肠道病毒核酸。结果:2010年4月-2010年11月共检测标本101份,病毒核酸检测阳性50份(49.50%),其中EV71为17.82%;CA16为19.80%;其他肠道病毒阳性为11.88%。101例标本中,从发病至采样0 d^2 d病毒核酸检出率最高(59.68%),其次是3 d^5 d(39.29%)。咽拭子、肛拭子或大便、疱疹液阳性率分别为48.42%、50.00%和100%。结论:黔东南州2010年手足口病流行毒株以肠道病毒CA16和EV71为主。

Objective：To understand the main pathogens of Hand-foot-mouth disease（HFMD）in Qiandongnan Prefecture,Guizhou in 2010.Methods：To collect 101 specimens,such as throat swabs,anus swabs or stools,and fluids of herpes of patients,detection of pathogens such as Enterovirus EV71 and CA16 are conducted by the RT-PCR method amplifying the virus specific fragments.Results： A total of 101 samples were detected from April to November 2010,of which,50 were positive.The positive rate of Enterovirus was 49.50%,including EV71（17.82%）,CA16（19.80%）,and others（11.88%）.The positive rate of samples collected within 0~2 days and within 3~5 days of onset of disease was 59.68% and 39.29%.The positive rate of throat swabs,anus swabs or stools,and fluids of herpes was 48.42%,50.00% and 100% respectively.Conclusion： The main pathogen that caused HFMD were EV71 and CA16 in Qiandongnan Prefecture in 2010.