通心络对高血压、高血糖合并高胆固醇血症大鼠动脉的影响及机制

Effects and mechanism of Tongxinluo on arteries of rats with hypertension,hyperglycemia and hypercholestero-lemia

目的评价通心络对高血压、高血糖合并高胆固醇血症大鼠模型动脉功能的影响及机制。方法将体重300～400 g的成年自发性高血压大鼠(SHR)32只随机分为普食组(SHR组)、对照组(模型组)、低剂量通心络(0.2 g/kg)干预组(通心络低组)、高剂量通心络(0.8 g/kg)干预组(通心络高组),另取8只正常血压大鼠作为正常组,高脂喂养4周建立动物模型。通心络干预4周后,行主动脉离体血管功能检查,用荧光定量PCR方法测定过氧化物酶体增殖物受体γ(PPAR-γ)mRNA表达。结果通心络干预后大鼠离体主动脉对乙酰胆碱、硝普钠引起的舒张反应增强,通心络高组对A23187的舒张反应增强(P<0.05),通心络剂量依赖性增加PPAR-γ基因表达。结论通心络干预具有保护血管内皮功能及逆转血管平滑肌功能障碍等作用,其机制可能与激活PPAR-γ基因表达有关。

Objective To study the effects and mechanism of Tongxinluo on arteries of rats with hypertension, hyperglycemia and hypercholesterolemia. Methods Adult spontaneously hyperteasive rats （SHR, weighing 300 N 400 g） were randomly divided into 4 groups： SHR group （n = 8, with normal diet）, model group （n =8, with high fat diet）, low - dose Tongxinluo （LT） group （ n = 8, treated with Tongxinluo of 0. 2 g/kg） and high - dose （HT） group （ n = 8, treated with Tongxinluo of 0. 8 g/kg）. Meanwhile, 8 Wistar rats fed with normal diet were set as normal control group. SHR model was constructed with high cholesterol diet for 4 weeks. Acetylcholine, A23187 and sodium nitroprusside were applied for artery ring study. RT - PCR was performed of quantification of peroxisome proliferator - activated receptor gam- ma （PPAR - γ） gene expression. Results Comparing with model group, significant magnification of cumulative concen- tration - dependent relaxation to acetylcholine and sodium nitroprusside was observed both in LT and HT groups, while significant amplified relaxation of A23187 was only observed in HT group （P 〈 0. 05 ）. The gene expression of PPAR γ was significantly increased by Tongxinluo follow dose - dependently manners. Conclusion Tongxinluo superfine powders pro- tect aorta endothelial function and reverse disturbances of smooth muscle cells, probably via induction of gene expression of PPAR - γ.