转笼康复训练对局灶性脑梗死大鼠旷场行为学和梗死灶周围Nogo-A表达的影响

The effects of cage-rotor rehabilitation training on the open-field behavior and the expression of Nogo-A around the infarct zone in rats with focal cerebral infarction.

目的研究转笼康复训练对局灶性脑梗死大鼠旷场行为学和脑梗死灶周围皮质Nogo-A表达的影响。方法将64只采用Longa颈外动脉线栓法制备的大脑中动脉闭塞SD大鼠随机分为康复训练组和造模对照组,每组32只。康复训练组大鼠每天进行滚筒训练40 min,造模对照组置于普通笼中自由活动。两组分别于造模后7、14、21和28 d 4个时间点随机选取8只大鼠进行旷场实验行为学分析后处死,取脑组织使用免疫组织化学方法观察脑梗死灶周围皮质Nogo-A的表达。结果康复训练组术后21、28 d旷场运动路程与造模对照组同时点相比差异具有统计学意义(P<0.05)。术后14、21、28 d康复训练组大鼠脑梗死灶周围皮质Nogo-A阳性细胞数与造模对照组同时点比较,差异亦具有统计学意义(P<0.05)。结论转笼康复训练能降低大鼠脑梗死灶周围Nogo-A的表达,减少其对神经轴突的抑制,促进脑梗死后的神经再生,增加中枢神经系统的修复能力。

Objective To study the effects of cage - rotor rehabilitation training on the open - field behavior and the expression of Nogo - A around the infarct zone in rats with focal cerebral infarction. Methods Middle cerebral artery occlusion （MCAO） was established on 64 Sprague - Dawley rats used Longa method. All rats were randomly divided into two groups ： cage - rotor rehabilitation training group （ n = 32 ） , in which 40 minutes rolling exercises everyday 48 hours after MCAO was given to subjects; control group （n = 32） , in which no special training exercises was given to subjects. Open - field behavior of random 8 rats each from both groups was tested on 7th, 14th, 21st and 28th day. After testing, rats were sacrificed for brain tissues for detection of expression of Nogo - A in peri- infarction region using immunohisto- chemistry. Results Significant different performance in open - field test was revealed between the two groups on 21 st and 28th day （ P 〈0.05）. Meanwhile, significant difference in expression of Nogo - A cells in peri - infarction region was ob- served between the two groups on 14th, 21st day and 28th day （P 〈 0. 05）. Conclusion The cage -rotor rehabilitation training reduces the expression of Nogo - A in peri - infarction region, reduces inhibiton on axon, and promotes nerve re- generation, resulting in improved recovery of the central nervous system.