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米诺环素对脑缺血再灌注损伤后炎性反应的影响 被引量：4

Effects of minocycline on brain inflammation of rat focal cerebral ischemia-reperfusion injury
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摘要 目的探讨米诺环素对局灶性脑缺血再灌注损伤后炎性反应的影响。方法采用线栓法制作大鼠大脑中动脉缺血再灌注模型。设定假手术组,生理盐水组,米诺环素预处理组、30 min组、4 h组。用免疫组织化学法、逆转录聚合链式反应技术、放射免疫法和核磁共振T2WI扫描等方法,观察米诺环素对缺血再灌注大鼠脑组织环氧化酶2(COX-2)、白细胞介素-1β转化酶(ICE)的表达,前列腺素E2(PGE2)的含量及脑缺血体积的影响。结果米诺环素能降低缺血再灌注大鼠脑组织中PGE2的含量,减少COX-2、ICE的表达,缩小脑梗死体积。结论米诺环素对大鼠局灶性脑缺血再灌注损伤后炎性反应有抑制作用。 Objective To study the effects of minocycline on brain inflammatory response of focal cerebral ischemia reperfusion injury in rats. Methods Sprague - Dawley rats were randomly divided into sham - operation group, control group,, minocycline preconditioning group, 30 min group and 4 h group. Focal cerebral ischemia was produced by introduction of intraluminal nylon in rats. The expression of cyclooxygenase -2 （ COX -2）, interleukin - 1 β converting enzyme （ICE） and prostaglandin E2 （PGE2） was assessed with immunohistochemistry, reverse transcription polymerase chain reaction and radioimmunity. Meanwhile, MRI was performed to assess focal ischemia area in rat brains. Results Minoeycline significantly reduced the expression of COX - 2, ICE and PGE2, and facol ischemia area in the brains of rats （P 〈 0. 05）. Conclusion Minocycline inhibits brain inflammation of rat cerebral ischemia - reperfusion injury.
作者 熊建忠 易飞 陆兵勋
机构地区 江西省萍乡市人民医院神经内科 南方医科大学南方医院神经内科
出处 《广东医学》 CAS CSCD 北大核心 2011年第11期1399-1401,共3页 Guangdong Medical Journal
基金 江西省科学技术专项基金项目(编号:2010BSB01800)
关键词 米诺环素 脑缺血再灌注 炎性反应 minlcycline cerebral ischemia - reperfusion inflammatory response
分类号 R743.31 [医药卫生—神经病学与精神病学]
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参考文献13

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二级参考文献3

共引文献11

同被引文献43

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广东医学
2011年 第11期

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