水葫芦沼液对青菜生长及AsA-GSH循环影响的动态研究

Dynamic changes in the growth and the AsA-GSH circulation metabolism of Chinese cabbage treated with a biogas slurry of water hyacinth

以青菜为材料,研究了在青菜整个生长周期内,不同比例水葫芦沼液对青菜生长的影响及其体内AsA-GSH循环影响的动态变化。结果表明,使用不同比例沼液代替化肥对青菜的株高及生物量产生不同的影响,其中,以25%沼液替代化肥的处理效果最好,在不同的采样时期,其株高和生物量均显著大于对照,当沼液使用比例大于50%时,株高和生物量均随着沼液使用比例的增加而降低;处理后总量Vc、还原型抗坏血酸(AsA)和脱氢抗坏血酸(DHA)的变化趋势不同,25%沼液替代化肥处理45和60 d时,总量Vc与对照相比显著增加;AsA虽然有所增加(除了30 d时),但与对照差异不显著;DHA含量均显著大于对照。当沼液使用比例大于50%时,总量Vc、AsA和DHA与对照相比均显著降低;在AsA循环中的抗坏血酸过氧化物酶(APX)、抗坏血酸氧化酶(AAO)和脱氢抗坏血酸还原酶(DHAR)活性变化趋势相似,均随着处理时间增加呈先增加后降低的趋势,其中以25%沼液替代化肥处理30 d酶活性最高,而单脱氢抗坏血酸还原酶(MDHAR)活性则随着处理时间的增加而增加,以25%沼液替代化肥的处理效果最好;在还原型谷胱甘肽(GSH)循环中的氧化型谷胱甘肽(GSSG)和GSH含量随着处理时间的增加而增加,以25%沼液替代化肥的处理效果最好,与对照相比差异显著,而谷胱甘肽还原酶(GR)活性随着处理时间增加呈先增加后降低的趋势,其中以25%沼液替代化肥处理45 d酶活性最高。说明用适量的水葫芦沼液替代化肥对青菜进行处理,有助于植株的生长,同时增加了体内AsA-GSH代谢循环,提高了青菜的抗氧化防御能力。

Dynamic changes in the growth and the AsA-GSH circulation metabolism of Chinese cabbage treated with different contents of biogas slurry of water hyacinth were studied in a complete growth cycle.Different responses were found in shoot height and fresh weight when the Chinese cabbages were treated with different contents of biogas slurry.The most obvious effect was observed with 25% biogas slurry.The shoot height and fresh weight significantly increased compared with those of the controls during different sampling periods.However,when the contents of biogas slurry were more than 50%,the shoot height and fresh weight were increasingly reduced with increases of biogas slurry contents.The contents of total Vc,reduced ascorbate（AsA） and dehydroascorbate（DHA） changed differently.With the 25% biogas slurry treatment,total Vc content significantly increased after 45 and 60 d treatment and AsA content also increased even though there was no significant difference compared with that in the controls.DHA content increased considerably during the different sampling periods.When the contents of biogas slurry were more 50%,the contents of total Vc,AsA and DHA were considerably reduced with increases of biogas slurry contents.In the AsA cycle,the activities of ascorbic acid peroxidase（APX）,ascorbate oxidase（AAO） and dehydroascorbate reductase（DHAR） showed the same change trend,initially increasing and then decreasing with an increase of treatment duration,i.e.the activities showed the maximum values after 30 d under 25% biogas slurry,whereas the activity of monodehydroascorbate reductase（MDHAR） increased with increased treatment duration.At the same time,the contents of oxidized glutathione（GSSG） and reduced glutathione（GSH） increased significantly with an increase of treatment duration in the GSH cycle.There were significant differences compared with those in the control.The activity of glutathione reductase（GR） increased initially and then decreased with an increase of treatment duration.The activity showed a maximum value after 45 d treatment with the 25% biogas slurry.It is suggested that the optimum biogas slurry content promotes growth,,enhances AsA-GSH circulation metabolism,and maintains higher antioxidative stress capacity in Chinese cabbage.