摘要
本试验采用流式细胞仪,对3个基因型8个继代周期的苜蓿愈伤组织DNA含量进行测定。通过DPAC软件(data pool application of cytometre)分析出细胞DNA含量变异百分率和处于S期细胞的百分率,并采用SPSS 10.0软件分析出愈伤组织继代时间与DNA含量变异率之间的关系。结果表明,本试验所采用的3个基因型8个继代周期24个紫花苜蓿愈伤组织样本DNA含量均发生了变化,且全都出现了DNA加倍的现象。在24个样本中,DNA含量变异率最大的是自选系大叶0510的愈伤组织继代15周时的样本,变异率达到了19.45%。通过SPSS10.0软件分析,表明紫花苜蓿愈伤组织DNA含量变异细胞百分率与继代时间存在相关性,随着继代时间的延长,染色体变异率有增加的趋势,但这种趋势不是无限增大的。继代0~12周是愈伤组织DNA含量变异的集中发生期,以后随着继代次数的增加,DNA含量变异率趋向于稳定。
DNA contents of callus of three genotype and eight culture time,which derived from anther culture in Medicago sativa,were detected by flow-cytometric analysis.The percentage of S stage cells and percentage of DNA content variation cells were analysed by the Data Pool Application of Cytometre software,the relationship between the callus cultivating time and the DNA content variation were analysed by the SPSS 10.0 software.One purpose of the study is to seek the relationship between DNA content variation and the culture time,and another is to provide the basic study of the law of DNA content variation in M.sativa for further research.DNA content of 24 samples were all changed and all DNA doubled.By the SPSS 10.0 software analysed,the correlativity between percentage of DNA content variation cells and cultivating time in M.sativa were found.With the increase of cultivating time,chromosome mutation were added.However,the trend was not always unchanged.The 0-12 week was the concentrated occurring stage of M.sativa callus DNA content variation,whereafter,DNA content variation become smooth with the increase of subculture times.
出处
《草业学报》
CSCD
北大核心
2011年第3期156-161,共6页
Acta Prataculturae Sinica
基金
国家自然科学基金(30671486)
国家863计划项目(2008AA10Z149)资助
关键词
苜蓿
花药愈伤组织
流式细胞仪
DNA含量变异
遗传变异
Medicago sativa
callus from anther culture
flow cytometry
DNA content variation
genetic variation