灯盏细辛对大鼠慢性高眼压模型视神经损伤保护的实验研究

Studies on the neuroprotective effects of Erigeron brevicapas hand mass on the optic nerve of chronic glaucoma model in rats

目的:建立大鼠慢性高眼压模型,观察灯盏细辛(Erigeronbrevicapas hand mass,EBHM)对眼压升高诱导视神经损伤的保护作用。方法:选用健康成年Wistar大鼠90只,分为3组。用波长为532nm的氪离子黄绿激光光凝第1和第2组大鼠双眼小梁网,建立大鼠高眼压模型。在眼压升高后1wk开始用EBHM对第2组大鼠15mg/100g肌肉注射,行视神经保护性治疗。第1组作为光凝对照组,第3组大鼠作为正常对照组。在第9wk同时处死3组大鼠做全视网膜铺片,1%甲苯氨蓝染色,记数平均视网膜神经节细胞(retinalganglion cells,RGCs)密度。结果:所有光凝眼眼压均中等程度升高,光凝前眼压为14.70±3.2mmHg;光凝后第3,6,9wk眼压分别为27.25±4.75,28.75±6.24,25.47±5.60mmHg,与光凝前比较,差别有显著性(P<0.05)。经视网膜铺片甲苯胺蓝染色,视网膜RGCs密度值(个/mm2)为:第1组1654±136,第2组2135±125,第3组2516±196。第2组大鼠视网膜RGCs密度值与第1和第3组大鼠RGCs密度值比较,差别有显著性(P<0.05)。结论:光凝大鼠小梁网成功建立大鼠慢性高眼压模型,光凝眼眼压中等程度升高,RGC密度降低;EBHM能够部分保护大鼠慢性高眼压诱导的视神经损害。

AIM：To investigate neuroprotective effects of Erigeron brevicapas hand mass（EBHM） on the optic nerve of chronic glaucoma model in rats. METHODS：Ninety adult Wistar rats were divided into three groups. The trabecular meshwork of both eyes of the 1st and 2nd group rats were performed photocoagulation with 532nm Krypton laser to establish chronic glaucoma model. Intraocular pressure（IOP） was measured by Tono-pen before and after surgery every week. After one week, EBHM was injected by 15mg/100g once a day in the 2nd group. The 3rd group acted as a control. All rats were killed at the 9th week. Retinal ganglion cells（RGCs） were counted based on the the wholemounted retinas stained with 1% toluidine blue. RESULTS：IOP of all the experimental eyes was increased. IOP was 14.70±3.2mmHg before operation. IOP of the experimented eyes was 27.25±4.75mmHg, 28.75±6.24mmHg, 25.47±5.60mmHg at the 3rd, 6th and 9th week respectively, there was a significant difference compared with preoperation. The value of the density of the RGCs was 1654±136 （per square millimeter ）in the 1st group rat eyes and was 2135±125 in the 2nd group rats, was 2516±196 in the 3rd group basing on the wholemounted retinas stained with 1% toluidine blue.There was a significant difference between eyes treated with EBHM and the eyes in other 2 groups in the value of the density of the RGCs. CONCLUSION：The IOP of the experimental rat eyes was chronically and moderiately increased by a laser injury to the trabecular meshwork,the value of the density of the RGCs was declining.EBHM can partly afford neuroprotection on the optic damage induced by pressure.