慢性肾功能衰竭患者血清对红系祖细胞的抑制及红细胞生成刺激素的疗效

GROWTH INHIBITION OF ERYTHROID COLONIES BY AUTOLOGOUS SERA AND THE CLINICAL EFFECT OF ERYTHROPOIETIN IN CHRONIC RENAL FAILURE

本文探讨了慢性肾功能衰竭患者自身血清对骨髓红系祖细胞的抑制作用及红细胞生成刺激素(erythropoietin EPO)的临床疗效。自身血清加入培养体系与胎牛血清加入组比较,半数病例集落数减少,自身血清用活性炭吸附后,这种造血抑制作用被解除。EPO治疗后的血色素增加程度和自身血清的造血抑制活性呈相关性,可见,如果能设法有效地去除患者体液中的造血抑制因子,EPO的临床疗效就能进一步提高。

We investigated the inhibitory effect of autologous sera on erythroid colony forimation(CFU-E) of bone marrow cells from patients with chronic renal failure and the clinical effect of recombinant erythropoietin, Colonies formed in cultures using autologous serum(AS) decreased in 15 cases as compared with those using fetal calf serum(FCS). This inhibitory effect of autologous sera was diminished by treatment with activated charcol in all these cases. The degree of hemoglobin increase after administration of recombinant erythropoietin appeared to correlate with the intensity of inhibitory activity of AS, These data indicate the clinical significance of an inhibitor(S) of erythropoiesis in uremic sera and suggest that the clinical effects of erythropoietin in this disease are further improved if the inhibitor(s) can be effectively removed.