摘要
目的建立马钱子散中士的宁的高效液相色谱测定方法。方法采用Akasil-C_(18)柱(200 mm×4.6 mm,5μm),以甲醇-0.01mol/L磷酸二氢钾溶液(25:75)为流动相,体积流量为1.0 mL/min,检测波长为254 nm。结果士的宁进样量在0.48～2.4μg与峰面积线性关系良好(r=0.999 5),平均回收率为98.9%,RSD为2.2%(n=6)。结论所建立的方法专属性强、准确、快速,适用于马钱子散中士的宁的测定。
Objeetive To develop a reversed-phase HPLC method for analyzing the content of strychnine in Maqianzi Powder. Methods The HPLC system comprised of an Akasil-C18 column （200mm×4.6mm,5μm）. The mobile phase was a mixture of methanol and 0.01 mol/L potassium dihydrogen phosphate solution （25 ： 75） and delivered at a flow rate of 1.0 mL/min. The detection wavelength was done at 254 nm. Results The calibration curve was found to be linear in injection amount range of 0.48--2.4μg （r = 0.999 5）. The mean recovery of strychnine was 98.9 % （RSD = 2.2%, n = 6）. Conclusion These results indicate that the HPLC method could be suitable for monitoring of strychnine in Maqianzi Powder since it is selective, accurate, and rapid.
出处
《现代药物与临床》
CAS
2011年第3期232-233,共2页
Drugs & Clinic
