大蒜素对白色念珠菌感染的免疫抑制小鼠IL-4和IgG产生水平的影响

Effects of allicin on the levels of IL-4 and IgG of immunosuppressed mice infected by Candida albicans

目的:探讨大蒜素对白色念珠菌感染免疫抑制小鼠体液免疫功能的影响。方法:将实验鼠随机分为预防组、治疗组和感染组。3组小鼠于实验前3 d腹腔一次性注射环磷酰胺200 mg/kg。实验时预防组小鼠灌服大蒜素7 d,其余两组灌服等量生理盐水;第8天各组小鼠尾静脉接种2×107/ml白色念珠菌菌液,同时给予预防组和治疗组分别灌服大蒜素7 d,感染组灌服等量生理盐水7 d;继之,收集3组小鼠脾细胞悬液和血清,测定其IL-4和IgG的表达。结果:与感染组相比,大蒜素可提高预防组和治疗组小鼠IL-4、IgG产生水平(P<0.01),且以预防组作用明显。结论:大蒜素可提高白色念珠菌感染模型鼠体液免疫的功能。

Objective：To explore the effects of allicin on humoral immune function of immunosuppressed mice infected by Candida albicans. Methods：The mice were randomized into groups of prevention., treatment and infection,and the animals in three groups were given single intraperitoneal administration of cyclophosphamide （CY） at dose of 200 mg/kg three days before the experiment. The mice in preventive group were administered orally with allicin for consecutive 7 days and two other groups, with equal amount of normal saline. On the 8th day, animals in three groups were inoculated with Candida albicarrs in concentration of 2 ×107/ ml via vena caudalis besides additional oral use of allicin in groups of prevention and treatment, and of physiological saline for the infected animals, for 7 days. Seven days later,the cultural liquid of spleen cells and serum of all mice were obtained for determining the level of IL-4 and IgG. Results：Allicin improved the levels of IL-4 and IgG of mice from both preventive and treatment groups as compared with the animals by infection of Candida albicans. Conclusion ： Alliein can boost the humoral immune function of the model mice infected by Candida albicans.