基于聚赖氨酸的星形嵌段共聚物对胰岛素的装载与控制释放研究

Investigation of Poly(L-lysine)-based Star-block Copolymers as pH-responsive Nanocarriers for Insulin

目的:研究合成的星形嵌段共聚物(PEI-PLL-b-mPEG)对胰岛素的装载以及pH敏感释放。方法:将PEI-PLL-b-mPEG与胰岛素混合体系对水溶液进行充分透析以测定装载量;将PEI-PLL-b-mPEG-胰岛素复合体对不同pH值的缓冲溶液进行透析,测定胰岛素的释放速率。结果:PEI-PLL-b-mPEG可实现对胰岛素的快速有效俘获并显示明显的pH敏感释放,同时能保持胰岛素的结构完整和生物活性。结论:合成的PEI-PLL-b-mPEG可用作酸度敏感释放的胰岛素载体。

Objective：To investigate the encapsulation and pH-responsive releasing properties of the synthesized star-block copolymers（PEI-PLL-b-mPEG）towards insulin.Methods：The PEI-PLL-b-mPEG-insulin mixture was dialyzed against PB buffer thoroughly to determine the loading capacities of insulin.The PEI-PLLb-mPEG-insulin complex was dialyzed against buffer solutions with different pH to determine the releasing profiles of the encapsulated insulin.Results：PEI-PLL-b-mPEG showed fast amd efficient encapsulation towards insulin,as well as pH-responsive releasing properties.The insulin released from PEI-PLLb-mPEG demonstrated retained chemical integrity and bioactivity.Conclusion： PEI-PLL-b-mPEG might have potential applications for controlled drug delivery.