两亲性刷状多肽共聚物纳米药物载体研究

Investigation on Amphiphilic Copolypeptide Brushes-based Drug Nanocarriers

目的:研究合成的两亲性刷状多肽共聚物(PLLF-g-(PLF-b-PLG))对疏水模型化合物(芘和油红)以及亲水模型化合物(结晶紫和阿霉素)的装载。方法:将PLLF-g-(PLF-b-PLG)与疏水模型化合物混合并充分搅拌、离心后,用分光光度法测定疏水模型化合物装载能力;将PLLF-g-(PLF-b-PLG)与阳离子亲水模型化合物混合体系对水溶液做充分透析除去自由模型化合物后,用分光光度法测定亲水模型化合物装载能力。结果:PLLF-g-(PLF-b-PLG)可分别有效稳定俘获疏水和阳离子亲水模型化合物,并且可实现两种模型化合物的共同装载。结论:合成的PLLF-g-(PLF-b-PLG)可用作疏水药物和亲水药物共同载体。

Objective：To investigate the encapsulation of the synthesized amphiphilic copolypeptide brushes（PLLF-g-（PLF-b-PLG））towards hydrophobic model compounds（pyrene and oil red）as well as cationic hydrophilic model compounds（crystal violet and doxorubicin）.Methods：The copolymer-hydrophobic model compound mixtures were fully stirred,centrifuged,and detected with UV-vis spectrophotometer to determine the loading capacity of hydrophobic model compounds.The cationic hydrophilic model compounds were mixed with the polymer,dialyzed thoroughly,and detected with UV-vis spectrophotometer to determine the loading capacity of hydrophilic model compounds.Results：PLLF-g-（PLF-bPLG）showed efficient and stable encapsulation towards hydrophobic and cationic hydrophilic model compounds,respectively,and could even encapsulate them simultaneously.Conclusion：PLLF-g-（PLF-b-PLG）can be used as potential nanocarriers for the simultaneous encapsulation of hydrophobic and cationic hydrophilic drugs.