中药当归及其混伪品的rDNA ITS序列分析与鉴别

rDNA ITS Sequence Analysis and Authentication of Angelica sinensis and its Adulterants

通过对当归及其混伪品中药材rDNA ITS序列的分析,并利用最大简约法构建系统树,试图寻找当归及其混伪品中药材rDNA ITS序列的差异和规律,建立当归与其混伪品药材之间的分子鉴别方法。结果表明：所有材料的ITS序列长度为598~601 bp。ITS1区总位点数为216,38个为简约信息位点（占17.6%）;ITS2区总位点数为225,31个为简约信息位点（占13.8%）。当归各个样品间遗传距离在0.000~0.003之间,当归与混伪品间遗传距离在0.074~0.135之间。当归与混伪品药材间的碱基差异显著,共有13个当归的特异鉴别位点,因此,rDNA ITS序列特征可作为当归及其混伪品药材鉴别的有效分子标记。

The rDNA ITS sequences of Angelica sinensis and its adulterants were analyzed in order to study the differences between A.sinensis and adulterants,and establish the molecular biological method for the identification of A.sinensis and the others.The results indicated that the length of ITS sequences varied from 598 to 601 bp.Of 216 total sites in ITS1,38 were parsimoniously informative（17.6%）;of 225 total sites in ITS2,31 were parsimoniously informative（13.8%）.The distance among 6 A.sinensis was 0.000 to 0.003.The distance between A.sinensis and its adulterants was 0.074 to 0.135.The differences in rDNA ITS regions between A.sinensis and their adulterants are obvious.13 distinct variable sites can be used as specific authenticable sites.Accordingly,the rDNA ITS sequence can be used as good marker for authenticating A.sinensis from its adulterants.