IFN-γ基因多态性与内蒙古汉族IgA肾病易感性关系的研究

The Study of IFN-γ Polymorphism and IgA Nephropathic Susceptibility in Inner Mongolia of Han Nationality

目的:探讨IFN-γ基因+874位点单核苷酸多态性与内蒙古汉族IgA肾病患者易感性的关系。方法:从131例内蒙古汉族IgA肾病的患者和138例健康对照者外周血中提取DNA,采用序列特异性引物聚合酶链反应(PCR-SSP)技术检测IFN-γ基因+874位点单核苷酸多态性。比较两组的基因型和等位基因的分布。结果:IgA肾病组中IFN-γ基因+874位点AA基因型频率和A等位基因频率显著高于正常对照组(P<0.05)。结论:IFN-γ基因+874位点单核苷酸多态性可能是IgA肾病的易感因素。

Objective To Study the Relationship between the single nucleotide polymorphism（SNP） in IFN-γ＋874 and IgA nephropathic susceptibility of the Han nationality patients in Inner Mongolia autonomous region.Methods Extract DNA from one hundred and thirty-one IgA nephropathy patients and 138 normal controls.The SNP in IFNγ＋874 gene were determined by special sequence primer of polymerase chain reaction（PCR-SSP）.IFNγ＋874 genetype and allele frequency were compared between patients with IgA nephropathy and normal controls.Results A significant difference of genetype and allele distribution in IFN-γ＋874 have been found between the patients with IgA nephropathy and healthy controls.Conclusion The SNP in IFNγ＋874 may be the predisposing factor for IgA nephropathy.