维甲酸调控线粒体途径对甲状腺滤泡状癌ASC的影响

The Effects of ASC of Mitochondrial Apoptosis Pathway Regulated by Retinoic Acid on the Growth of Follicular Thyroid Neoplasm

目的:研究维甲酸(retinoic ac id,RA)对甲状腺滤泡状癌细胞FIE-133ASC调控的影响,并探讨线粒体作用机制与凋亡相关斑点样蛋白(apoptosis-assoc iated speck like prote in conta in ing a CARD,ASC)的关系。方法:应用噻唑蓝(MTT)比色法检测RA在不同时间(12、24、36、48h)下对甲状腺滤泡状癌细胞FIE-133存活率的影响,透射电镜观察细胞线粒体超微形态结构的改变,蛋白印迹法检测经RA作用前后ASC蛋白的表达。结果:RA对FIE-133细胞生长的抑制有时间依赖性,电镜下甲状腺滤泡状癌细胞线粒体结果发生剧烈改变,ASC表达明显增加。结论:RA对甲状腺滤泡状癌细胞FIE-133的生长具有抑制作用,这一抑制作用是由于线粒体凋亡通路调节ASC表达的影响使细胞凋亡功能得以恢复。

Objective To investigate the role of ASC（apoptosis-associated specklike protein containing a CARD,ASC） in FIE-133 cell line of follicular thyroid neoplasm induced by retinoic acid,and the correlation of mitochondrial mechanism.Methods Methyl thiazolyl tetrazolium（MTT） method was used to measure the growth of FIE-133 cells treated with retinoic acid at different time points（12,24,36,48h）.The mitochondrial structure was observed by electron microscopic technique（EM）.ASC were detected by Western-blotting before and after treatment with retinoic acid.Results Acid inhibited FIE-133 cells in time-dependent manners after treatment with retinoic acid.The intensity of mitochondria were occurred through scanning and transmission electron microscope.The expression of ASC was up-regulated in FIE-133 cells treated with retinoic acid.Conclusion Retinoic acid had inhibitory effect for growth of FIE-133 cells line of follicular thyroid neoplasm.Mitochondrial apoptosis pathway might be regulated with ASC and recover cells apoptosis.