摘要
Ochrobactrum anthropi CTS-325 isolated from a chromium-contaminated site had better resistance to Cr（Ⅵ） in LB medium under aerobic condition.Meanwhile,it was found that the reduction of Cr（Ⅵ） is not complete during the experimental process.Therefore,a series of small molecule energy sources including nitrogen and carbon sources were added into the LB medium in the bacterial stationary phase to promote the chromium reducibility.The result showed that the bacterial growth was positively correlated with the chromium reduction.SDS-PAGE analysis indicated that the protein groups were changed when the bacteria were stimulated by the chromium.Additionally,it was revealed that O.anthropi CTS-325 could utilize the cheaper alternative of sugar（sucrose residue leaching solution） well for further growth and restart the chromium reduction,which offered a new method for practical appli-cations.
Ochrobactrum anthropi CTS-325 isolated from a chromium-contaminated site had better resistance to Cr（Ⅵ） in LB medium under aerobic condition.Meanwhile,it was found that the reduction of Cr（Ⅵ） is not complete during the experimental process.Therefore,a series of small molecule energy sources including nitrogen and carbon sources were added into the LB medium in the bacterial stationary phase to promote the chromium reducibility.The result showed that the bacterial growth was positively correlated with the chromium reduction.SDS-PAGE analysis indicated that the protein groups were changed when the bacteria were stimulated by the chromium.Additionally,it was revealed that O.anthropi CTS-325 could utilize the cheaper alternative of sugar（sucrose residue leaching solution） well for further growth and restart the chromium reduction,which offered a new method for practical appli-cations.
基金
Supported by the National Basic Research Program of China (973 program,No. 2007CB815601,2010CB933501)
the National Natural Science Foundation of China (40772034, 40902097)
the Outstanding Youth Fund (50625205)
the Opening Project of Key Laboratory of Solid Waste Treatment and Resource Recycle (No. 09zxgk05),Ministry of Education
the CAS Foundation (KJCX1.YW.07)
