CSTR和批式反应器中酶法拆分萘普生的比较

Comparison between Enzymatic Kinetic Resolution of Naproxen in Batch Reactor and CSTR

比较了批式反应器和连续流动搅拌反应器（CSTR）中酶动力学拆分萘普生的不同之处．从宏观反应器平衡角度，推导出了在CSTR中不同于在批式反应器中的酶立体选择性（E）、产物对映体过量值（eep）和反应转化率（ξ）的定量关系式．并通过脂肪酶催化的萘普生甲酯的不对称水解反应得到了证实．分别在CSTR和批式反应器中进行萘普生的酶法拆分，在一定的转化率下，从批式反应器中得到的产物的对映体过量值高于CSTR反应器的结果；相同条件下，批式反应器中的转化率也高于CSTR反应器中．连续操作1200h，批式反应器中剩余酶活力为65％，CSTR中剩余酶活力为80％，CSTR中酶的操作稳定性优于批式反应器．

The difference between a continuous stirred-tank reactor (CSTR) and a batchwise reactor has been shown for the enzymatic kinetic resolution of naproxen. By macroscopic reactor balancing, new quantitative relationships between enzyme enantioselectivity, product enantiomeric excess and the extent of conversion have been deduced for chiral resolution in a CSTR. They were experimentally verified by the lipase-catalyzed asymmetric hydrolysis of racemic naproxen methyl ester. The stereoseIective hydrolysis reaction was performed both in a batch system and a CSTR. At a particular conversion slightly higher enantiomeric excess of product can be obtained in a batch system. Under similar reaction conditions a higher conversion can be obtained in a batch system. After 1200 h continuous operation, the immobilized lipase running in a batch reactor reserves 65% of its original activity and running in a CSTR reserves 80%, that is, the immobilized lipase is more stabe in a CSTR.