正交试验法优选肝毒清胶囊的提取工艺

Optimization of extraction process of "GanDuQing" capsule by orthogonal design

探讨肝毒清胶囊处方中有效成分芍药苷、甘草酸、甘草苷的最佳提取工艺条件。采用正交试验,考察粉碎粒度,浸泡时间,加水量,煎煮时间,煎煮次数等因素对肝毒清胶囊处方中芍药苷、甘草酸、甘草苷提取率的影响,实验采用高效液相色谱法测定有效成分的含量。优化后的提取工艺为:甘草粒径为0.9—2mm,白芍为0.9—2mm,加16倍量的水,浸泡1 h,煎煮3次,每次2 h。在此提取条件下,提取量达16.893 mg/g,优选出的工艺可适用于工业化生产。

To study the optimization extraction process conditions of paeoniflorin and liquorice in＂GanDuQing＂capsule,we study the experimental conditions such as time and ratio of material-fluid influencing the extracting efficiency of White paeoniae alba and liquorice.The result is as follows,when the liquorice particlesize is 0.9-2mm,white Paeony Root is 0.9-2mm,ratio of material-fluid is 1∶16 and soaking time is 1h,extraction 3 times and each time is 2h,the total extraction rate of ＂GanDuQing＂ capsule is 16.893mg/g.The optimum extraction technology is scientific and reasonable,and it is suitable for industrial production.