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毕赤酵母表达系统的实际应用研究 被引量：1

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摘要 在DNA体外重组和异源表达技术中．酵母表达系统是重要的一个组成部分。对于它的优势，大量中外文献用实例证明，用综述总结。酵母菌是最简单的真核模式生物，兼有原核、真核两种表达系统的优点：
作者 刘春凤
机构地区 厦门伯赛基因转录技术有限公司
出处 《厦门科技》 2011年第3期51-56,共6页 Xiamen Science & Technology
关键词 毕赤酵母表达系统 应用 组成部分 异源表达 体外重组 模式生物 DNA 酵母菌
分类号 Q78 [生物学—分子生物学]
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参考文献10

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  • 2Graham G J,Maio J J.A rapid and reliable method to create tandem arrays of short DNA seguences. Biotechniques . 1992
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  • 5Scorer CA,Buckholz RG,Clare JJ,et al.The intracellular production and secretion of HIV-1 envelope protein in the methylotrophic yeast Pichia pastoris. Gene . 1993
  • 6Masayuki Yamada,Takeshi Azuma,Takao Matsuda,et al.Secretion of human intracellular aspartic proteinase cathepsin E expressed in the methylotrophic yeast and characterization of produced recombinant cathepsin E. Biochimica et Biophysica Acta . 1994
  • 7Otvos L Jr,Krivulka GR,Urge L,et al.Comparison of the effects of amino acid substitutions and beta-N-vs, alpha-O-glycostlation on the T-cell stimulatory activity and conformation of an epitope on the rabies virus glycoprotein. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research . 1995
  • 8Waterham H R,Digan M E,Koutz P J,et al.Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter. Gene . 1997
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同被引文献47

  • 1邓毛程,王瑶,冯文清,徐国华.搅拌器对谷氨酸菌高密度培养的影响[J].食品与机械,2007,23(5):101-103. 被引量：2
  • 2Gasser B, Prielhofer R, Marx H, et al. Picbia pastoris : protein production host and model organism for biomedical research. Future Microbiology, 2013, 8 (2): 191-208.
  • 3Mack M, Wannermacher M, Hob1 B, et al. Comparison of two expression platforms in respect to protein yield and quality: Pichia pastoris versus Pichia angusta. Protein Expression and Purification, 2009, 66(2): 165-171.
  • 4Staley C A, Huang A, Nattestad M, et al. Analysis of the 5' untranslated region (5' UTR) of the alcohol oxidase 1 ( AOX1 ) gene in recombinant protein expression in Pichia pastoris. Gene, 2012, 496 (2) : 118-127.
  • 5Zhou W J, Yang J K, Miao L H, et al. Codon optimization, promoter and expression system selection that achieved high-level production of Yarrowia lipolytica lipase in Pichia pastoris. Enzyme and Microbial Technology, 2015, 71 : 66-72.
  • 6LiL , Qian D M , Shao G G , et al. High-level expression , purification and study of bioactivity of fusion protein M-IL-2 (ss Arg , 123AIa ) in Pichia pastpris. Protein Expression and Purification , 2014 , 101 : 99-105.
  • 7Zhang J, Wang X, Zheng Y, et al. Enhancing yield of S- adenosylmethionine in Pichia pastoris by controlling NH4^+ concentration. Bioprocess and Biosystems Engineering, 2008, 31 (2) : 63-67.
  • 8Salunkhe S, Soorapaneni S, Prasad K S, et al. Strategies to maximize expression of rightly processed human interferon α2b in Pichia pastoris. Protein Expression and Purification, 2010, 71 (2) : 139-146.
  • 9Du C, Hart L, Xiao A, et al. Secretory expression and purification of the recombinant duck interleukin-2 in Pichia pastoris. Journal of Microbiology and Biotechnology, 2011, 21 (12) : 1264-1269.
  • 10Zhou X Y, Ying Y, Jianjun T, et al. Production of LYZLr, a novel human c-type lysozyme, in recombinant Pichia pastoris employing high cell density fed-batch fermentation . Journal of Bioscience and Bioengineering, 2014 ,1 18 (4) : 420-425.

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厦门科技
2011年 第3期

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