棉铃虫化学感受蛋白HarmCSP6二聚体的组织表达分析及气味结合特征

Expression profiling and binding characterization of dimeric chemosensory protein 6 (HarmCSP6) in Helicoverpa armigera (Hübner) (Lepidoptera:Noctuidae)

为了研究棉铃虫Helicoverpa armigera(Hübner)化学感受蛋白(chemosensory proteins,CSPs)在嗅觉识别过程中的功能,本研究克隆并在原核细胞中表达了棉铃虫化学感受蛋白HarmCSP6,Western blot和快速液相色谱(fast protein liquid chromatography,FPLC)检测证实该HarmCSP6蛋白以二聚体的形式表达。实时荧光定量PCR检测发现HarmCSP6基因在雌雄虫触角中表达量很高,在雌虫足及翅中也有一定程度的表达。该二聚体蛋白与22种气味分子的荧光竞争结合实验结果表明,该蛋白与醛类及萜烯类等气味小分子具有较强的结合能力。通过研究该蛋白与小分子的结合,筛选出合适的气味,从而为开发棉铃虫引诱剂及驱避剂提供理论依据。

In order to study the functions of chemosensory proteins （CSPs） of Helicoverpa armigera （Hübner） in olfactory recognition,we cloned and expressed the chemosensory protein HarmCSP6 of H.armigera in prokaryotic cell.This protein was expressed in the form of dipolymer,which was certified by Western blotting and fast protein liquid chromatography （FPLC）.The results of expression profiles showed that the HarmCSP6 gene was highly expressed in male and female antennae,and was expressed to a certain extent in female legs and wings.The binding experiment with 22 kinds of odorant chemicals indicated that HarmCSP6 could bind obviously with aldehydes and terpenes.By studying the binding affinity,some special binding odorants can be selected.This may provide the theoretical basis for developing attractants and repellents of the cotton bollworm.