组蛋白去乙酰化酶抑制剂对人外周血CD4^+ T细胞Th1/Th2分化的影响

Effect of trichostatin A and sodium butyrate on variation of Th1/Th2 cell balance after differentiation of peripheral blood CD4^+ T cells

目的探讨曲古抑菌素A(TSA)和丁酸钠(NaB)对人外周血CD4+T细胞向Th1/Th2分化的影响。方法体外分离并培养人外周血CD4+T细胞,分为TSA组(0.33μmol/L)、NaB组(1.4 mmol/L)和对照组(CON组)。采用MTT、流式细胞术、qRT-PCR和酶联免疫吸附试验(ELISA)法检测TSA组、NaB组及对照组细胞因子IFN-γ、IL-4表达水平及IFN-γ+细胞/IL-4+细胞比值。结果外周血CD4+T细胞在植物血凝素(PHA)刺激48 h后,ELISA检测细胞培养上清显示:TSA组、NaB组上清IFN-γ、IL-4分泌水平均明显高于对照组(P<0.05)。qRT-PCR检测显示:TSA组、NaB组IFN-γmRNA表达高于对照组,相比对照组提高到(3.12±0.34)、(2.3±0.51)倍,差异有统计学意义(P<0.05);IL-4 mRNA提高到(1.87±0.42)、(1.63±0.51)倍,差异有统计学意义(P<0.01)。流式细胞术对胞内因子检测显示:TSA组、NaB组IFN-γ+细胞/IL-4+细胞比值明显升高,是对照组的1.93、1.55倍,差异有统计学意义(P<0.01)。结论 TSA、NaB暴露可引起人外周血CD4+T细胞Th1/Th2分化平衡改变,出现向Th1方向的"Th1/Th2平衡的漂移"。

Objective To investigate the effects of trichostatin A（TSA） and sodium butyrate（NaB）, histone deacetylase inhibitors, on the balance of Thl/Th2 after differentiation of peripheral blood CD4＋T cells. Methods Peripheral CD4^＋T cells were grown in vitro and were divided into TSA group, NaB group, and CON group. The concentrations of IL-4 and IFN-γ （Thl/Th2 factors） in the supernatants were examined by ELISA in each group 48 h later; the expressions of IL 4 and IFN-γ were analyzed by qRT-PCR; and the Tdymphoeytes in peripheral blood （PBL） were measured by flow cytometry. Results ELISA showed that the supernatant IFN-γ and IL 4 levels in the TSA and NaB groups were significantly higher than those in the CON group 48 h after treatment with TSA（0.33 μmol/L） and NAB（1.4 mmol/L） ; real-time quantitative PCR also showed that the expressions of IFN-γ and IL-4 mRNA in TSA and NaB groups were significantly higher than those in the CON group （3.12 ： 1, 2.3 ： 1 ; P〈0.05）. However, flow cytometry showed that the ratios of IFN-γ^＋ cells/IL-4^＋ cells were significantly higher in TSA and NaB groups than that in the CON group（（1.93 ： 1,1.55 ： 1 ;P〈0.05） . Conclusion Treatment with TSA and NaB, both histone deacetylase inhibitors,can induce a shift of Thl/Th2 cell balance toward Thl cells in peripheral CD4^＋T cells.