液质联用技术在中药代谢物及代谢组学研究中的应用分析

Application of LC-MS technique in metabolite and metabonomic research of traditional Chinese medicine

中药和天然产物在生物体内通常发生广泛代谢,因其代谢途径的复杂性以及多组分、多靶点、整体调节作用的特点,现已将代谢组学应用于中药代谢产物及其药效机制的研究中。但是,由于代谢组学研究要求获得生物体液中大量外源性与内源性代谢物信息,这就对分析技术提出了很高的要求。本文综述了中药代谢物和代谢组学研究中常用的液质联用技术,并对PubMed检索到的23篇相关文献进行数据归纳与比较分析,重点讨论不同种类液质联用技术在中药代谢组学研究应用中的优缺点,指出UPLC-Q-TOF/MS的优势,以期为中药代谢组学研究提供新思路。

Traditional Chinese medicine （TCM） and natural products are widely metabolized in organism. Due to the complexity of their metabolic pathways and ＂multiple components, multiple targets and the overall regulation functions＂ of TCM and natural products in organism, metabonomics have been widely applied in researches on TCM metabolites and pharmacodynamic mechanism. However, metabonomics study needs the information of large amount of exogenous and endogenous metabolites in the biological fluids, which requires more powerful analytical techniques. This paper reviewed liquid chromatography coupled with mass spectrometry （LC-MS） techniques commonly applied in TCM metabolite and metabonomics researches. We summarized and comparatively analyzed the data of 23 literatures we retrieved from Pubmed database. We focused on the advantages and disadvantages of various LC-MS techniques in TCM metabonomics studies; the advantages of UPI.C-Q-TOF/MS were introduced in a detained manner, hoping to cast new lights on TCM metabonomics researches.