麦冬多糖MDG-1对非糖尿病小鼠糖耐量及肠道菌群的影响

Effects of polysaccharide MDG-1 from Ophiopogon japonicus on glucose tolerance and intestinal flora in non-diabetic mice

目的探讨麦冬多糖MDG-1对正常小鼠的糖耐量作用及肠道菌群的影响。方法 KM小鼠随机分为9组:淀粉组、淀粉+MDG-1组、淀粉+阿卡波糖组,葡萄糖组、葡萄糖+MDG-1组、葡萄糖+阿卡波糖组,蔗糖组、蔗糖+MDG-1组、蔗糖+阿卡波糖组。灌胃给药阿卡波糖400 mg·kg^(-1)·d^(-1),麦冬多糖MDG-1 300 mg·kg^(-1)·d^(-1),单次给药2 h及连续给药2 wk后行口服糖耐量实验。另设空白组、MDG-1低剂量(150 mg·kg^(-1)·d^(-1))组、MDG-1高剂量(300 mg·kg^(-1)·d^(-1))组,连续给药3 wk后,对小鼠肠道菌群进行培养,观察小鼠肠道菌群的变化情况。结果单次给药糖耐量实验结果显示,淀粉+MDG-1组AUC_(0-120min)低于淀粉组(P<0.01)、蔗糖+MDG-1组AUC_(0-120min)低于蔗糖组(P<0.05),葡萄糖负荷各组血糖无显著差异(P>0.05)。连续给药糖耐量实验结果显示,蔗糖+MDG-1组和葡萄糖+MDG-1组糖负荷后AUC_(0-120min)分别低于蔗糖组、葡萄糖组(P<0.05),淀粉负荷各组血糖无显著差异(P>0.05)。MDG-1高剂量组肠道乳酸杆菌和双歧杆菌数高于空白组,差异显著(P<0.05),大肠杆菌和链球菌数量低于空白组,但无显著差异(P>0.05)。结论麦冬多糖MDG-1对非糖尿病小鼠的糖耐量有显著影响,同时对肠道微生态有一定的调节作用。

AIM To investigate the effects of polysaccharide MDG-1 from Ophiopogon japonicus on ucose tolerance and intestinal flora in non-diabetic mice. METHODS Fifty-one male KM mice were randomly divided into starch group, starch ＋ MDG-1 group, starch ＋ acarbose group, glucose group, glucose ＋ MDG-1 group, glucose ＋ acarbose group, sucrose group, sucrose＋ MDG-1 group and sucrose ＋ acarbose group, and were administered respectively with acarbose 400 mg ·kg^-1·d^-1 and polysaccharide MDG-1 300 mg·kg^-1·d^-1 by gavage once daily. Glucose tolerance test was performed after a single dose of the drug and 2 wk after the continuous administration of the drug. Another 27 mice were randomized into control group, MDG-1 low dose group （150 mg·kg^-1·d^-1） and MDG-1 high dose group （300 mg·kg^-1·d^-1）. The changes in intestinal flora were investigated 3 wk after the continuous administration of polysaccharide MDG-1. RESULTS After a single dose of the drug, AUCe-120min in starch ＋ MDG-1 group was lower than starch group （P 〈 0.01）, AUC0-120min, in sucrose ＋ MDG-1 group was lower than sucrose group （P 〈 0.05）. AUC0-120min, in sucrose ＋ MDG-1 group and glucose ＋ MDG-1 group were lower than sucrose group and glucose group, respectively, 2 wk after the continuous administration of the drug. Compared with the control group, the number of lactobacilli and bifidobacteria were increased in MDG-1 high dose group （P 〈 0.05） , the number of E. coli and streptococci were decreased in MDG-1 high dose group, but there was no significant difference （P 〉 0.05）. CONCLUSION Polysaccharide MDG-1 from Ophiopogonjaponicas exerts a remarkable influence on glucose tolerance in non-diabetic mice, and has a certain regulatory effects on intestinal flora.