平肝潜阳方药对自发性高血压大鼠血管组织蛋白质表达谱的影响

Changes of protein expression profile in vascular tissues of spontaneously hypertensive rats treated by a compound Chinese herbal medicine

目的:观察平肝潜阳方药对自发性高血压大鼠血管组织中蛋白质表达的影响,为深入研究平肝潜阳方药治疗高血压的作用机制奠定基础。方法:选择WKY大鼠10只作为正常对照,自发性高血压大鼠20只随机分为模型组和平肝潜阳方治疗组。治疗时间为4周,治疗期间每周监测大鼠血压和心率,并观察大鼠行为学指标;治疗结束后,采用双向凝胶电泳(two-di mensional gel electrophoresis,2-DE)结合质谱技术分离鉴定血管组织相关蛋白。结果:平肝潜阳方药不仅能够明显降低自发性高血压大鼠的血压,而且能够改善大鼠的心率、易激惹程度和旋转耐受时间。本实验建立了自发性高血压大鼠血管组织的2-DE图谱。与正常组比较,模型组蛋白质点表达上升2倍及以上的有12个,下降2倍及以上的有15个。治疗组与模型组比较发现,表达下调的15个蛋白质中有10个表达增强,而表达上调的12个蛋白质中有8个表达降低。共得到16个肽质量指纹图谱,3个无质谱结果,搜索到13个具有统计学意义的蛋白质。结论:平肝潜阳方药能够降低血压和改善大鼠的行为学指标,其机制可能与调节血管组织的蛋白质表达谱有关。

Objective：To investigate the effects of a Chinese herbal formula for calming liver and suppressing yang on the protein expressions of vascular tissues in spontaneously hypertensive rats（SHRs）,and to explore the mechanism of efficacy.Methods：Twenty SHRs were randomly divided into model group and treatment group.Another 10 Wistar-Kyoto rats were selected as a normal control.SHRs in the treatment group were administered with the formula for calming liver and suppressing Yang for 4 weeks.During the course of treatment,blood pressure and heart rates were monitored every week and the ethology of rats,including irritability and rotation endurance was also evaluated.After treatment,thoracic aorta was obtained and its proteins were separated by 2-dimensional gel electrophoresis（2-DE）.The differentially expressed proteins were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF MS）and database query.Results：The formula for calming liver and suppressing yang not only decreased the systolic blood pressure and heart rate,but also improved irritability degree and rotation endurance time of SHRs.This experiment had established the 2-DE graph of protein expressions of vascular tissues in SHRs.Compared with the normal group,the expressions of 15 proteins were down-regulated,and 12 proteins were up-regulated in vascular tissues of the model group.The formula for calming liver and suppressing yang treatment up-regulated expressions of 10 proteins in the 15 down-regulated proteins,and down-regulated 8 proteins in the 12 up-regulated proteins in vascular tissues of SHRs.After analysis,16 obviously differentially expressed proteins were found,and 13 of them were identified.Conclusion：The formula for calming liver and suppressing yang can improve the ethology of SHRs.The mechanism is probably concerned with regulating the protein expressions of vascular tissues.