富铬松花粉中铬元素的含量测定及降血糖作用研究

Chromium content determination and hypoglycemic effect in the chromium-enriched pine pollen

目的测定富铬松花粉中铬元素的含量,探讨富铬松花粉对高血糖大鼠的作用和对大鼠糖耐量的影响。方法 HNO3-HC lO4湿式消化法处理有机铬样品,采用原子吸收分光光度法(AAS)检测富铬松花粉中铬元素的含量;尾静脉注射四氧嘧啶诱导高血糖大鼠模型,连续灌胃给药15 d后,葡萄糖氧化酶-过氧化物酶法检测血糖含量;正常大鼠连续灌胃给药10 d后,灌胃葡萄糖溶液(2.0 g·kg-1),于给糖前及给糖后30、60、120 m in检测血糖含量变化。结果采用原子吸收分光光度计测定铬的标准曲线为A=0.009 8C+0.006 2,测得三批富铬松花粉中铬元素的含量分别为16.775 6、17.682 9、17.988 3μg·g-1。富铬松花粉(1.12 g·kg-1)显著降低四氧嘧啶诱导高血糖大鼠血糖水平。富铬松花粉(1.12、0.56 g·kg-1)显著降低正常大鼠血糖水平,富铬松花粉(0.56 g·kg-1)在120 m in时降糖作用明显。结论三批富铬松花粉中铬元素的平均含量为17.482 2μg·g-1;富铬松花粉具有降血糖的作用。

Aim To determine content of chrominum in the chromium-enriched pine pollen,and to explore the hypoglycemic effect of the chromium-enriched pine pollen and sugar tolerance in rats.Methods HNO3-HClO4 Wet Digestion was used to process organic chromium sample,and the content of chromium in the chromium-enriched pine pollen was determined by Atomic Absorption Spectrophotometry（AAS）;Model of diabetic rats was established by caudal vein injection of alloxan.After a 15-day intragastric administration of the chromium-enriched pine pollen on these rats,glucose oxidase-peroxidase method was used to determine the glucose level in the blood,while after having received successive intragastric administration of the chromium-enriched pine pollen for 10 days,changes of blood sugar levels of normal rats were determined pre and post 30,60,120 mins respectively after intragastric administration of glucose solution at the dose of 2.0 g·kg-1.Results The standard curve of chrominum,tested by Atomic absorption spectrophotometry,is A=0.009 8C＋0.006 2;three batches of chromium-rich Pollen content of chromium measured were 16.775 6,17.682 9,and 17.988 3 μg·g-1 respectively.The chromium-enriched pine pollen（1.12 g·kg-1）significantly reduced the blood sugar levels of diabetic rats induced by alloxan.The chromium-enriched pine pollen（1.12 g·kg-1）significantly reduced the blood sugar levels of normal rats as well,especially remarkable in hypoglycemic effect at 120 min with the chromium-enriched pine pollen（0.56 g·kg-1）.Conclusion The average chromium content of three batches of chromium-rich pine pollen is 17.482 2 μg·g-1,and the chromium-enriched pine pollen has the hypoglycemic effect.