HPLC法测定柴胡口服液中柴胡皂苷a、d的含量

Determination of Saikosaponin a,d in oral bupleurum by HPLC

目的 建立测定柴胡口服液中柴胡皂苷a、d含量的方法.方法 用高效液相色谱法测定制剂中柴胡皂苷a和d的含量.色谱柱：Welchrom C18（4.6 mm×250 mm,5 μm）,流动相：乙腈-水（43：57）,检测波长：210 nm;流速：1.0 ml·min-1.结果 柴胡皂苷a线性范围为1.962～11.772 μg,r＝0.999 8,平均回收率99.66%,RSD为0.10%;柴胡皂苷d线性范围为2.772～16.632 μg,r＝0.999 9,平均回收率99.68%,RSD为0.28%.结论 该方法操作简便、灵敏度高、结果准确、专属性强,能有效的控制柴胡口服液的质量.

Aim To determine saikosaponin a,d in Oral Bupleurum.Methods HPLC was used for the determination of saikosaponin a,d content.Welchrom C18（4.6 mm×250 mm,5 μm）was used and the mobile phase was acetonitrile-water（43∶57）.The detective wavelength was set at 210 nm.The flow rate was 1.0 ml·min-1.Results The linear range of saikosaponin a and saikosaponin d were 1.962～11.772 μg（r=0.999 8）and 2.772～16.632 μg（r=0.999 9）.The average recovery rate and RSD were 99.66%,99.68%and 0.10%,0.28%.Conclusion The method is simple,sensitive,accurate,specific,and can effectively control the quality of Oral Bupleurum.