暗紫贝母愈伤组织和不定芽诱导研究

Research on induction of Callus and Adventitious Buds from F.unibracteata

目的:对暗紫贝母愈伤组织和不定芽诱导进行研究。方法:以暗紫贝母新鲜鳞茎为外植体,在附加NAA和6-BA不同浓度配比的MS培养基上进行离体培养。结果:鲜鳞茎能诱导形成愈伤组织和不定芽。其中,NAA1.2mg/L+6-BA1.8mg/L的MS培养基中外植体愈伤组织诱导率最高,达53.3%;NAA1.2mg/L+6-BA1.6mg/L的MS培养基中外植体不定芽诱导率最高,达73.3%。结论:建立了暗紫贝母愈伤组织和不定芽诱导的培养方法。

Objictive： Study on the induction of callus and adventitious buds from F.unibracteata. Methods： Explants from the fresh bulb-scale sections of F.unibracteata were in-vitro cultured using Murashige and Skoog （MS） medium supplemented with different ratios concentration of NAA and 6-BA. Results： The fresh bulb-scale sections could be induced to the callus and adventitious buds. The callus induction rate of the explants in MS medium with NAA（1.2 mg/L） and 6-BA（1.8 mg/L） was the highest, reaching 53.3%. The adventitious buds induction rate of the explants in MS medium with NAA（1.2 mg/L） and 6-BA（1.6 mg/L） was the highest, reaching 73.3%. Conclusion： Th is study established the culture methods of callus and adventitious buds from F.unibracteata.