基质金属蛋白酶-9和上皮型钙依赖黏附素在食管癌中的表达及其意义

Expression of MMP-9 and E-cadherin in esophageal carcinoma and its significance

目的探讨基质金属蛋白酶-9(MMP-9)和上皮型钙依赖黏附素(E-cadherin)在食管癌中的表达及其意义。方法应用免疫组化SP法检测69例食管癌组织及69例正常者胃镜取材组织中MMP-9和E-cadherin蛋白表达情况。结果食管鳞癌组织中MMP-9的阳性表达率高于正常食管黏膜鳞状上皮组织(71.0%vs46.4%,P<0.05),食管鳞癌组织中E-cadherin的阳性表达率低于正常食管黏膜鳞状上皮(50.7%vs72.5%,P<0.01)。MMP-9与E-cadherin的阳性表达率在食管癌不同病理分级及有无淋巴结转移中差异均有统计学意义(P<0.05或P<0.01);随着病理分级的增高及MMP-9阳性表达率依次增强,而E-cadherin阳性表达率依次降低(P<0.05或P<0.01);浸润达深肌层组E-cadherin蛋白阳性表达率低于浸润达浅肌层组(P<0.01);MMP-9与E-cadherin的蛋白表达呈负相关(r=-0.269,P<0.05)。结论 E-cadherin、MMP-9表达可能与食管癌的发生发展密切相关。

Objective To investigate the expressions of Matrix metalloproteinases-9（MMP-9） and E-cadherin in esophageal carcinoma and its significance.Methods The expression levels of MMP-9 and E-cadherin proteins in cancer tissues of 69 patients with esophageal carcinoma and normal esophageal squamous epithelial tissues of 69 control subjects were determined by immunohistochemical analysis（SP method）.Results The expression level of MMP-9 was significantly higher in esophageal carcinoma tissues than that in normal tissues（71.0% vs 46.4 %,P〈0.05）,and the positive expression rate of E-cadherin in esophageal carcinoma tissues was significantly lower than that in normal esophageal mucous epithelial tissues（50.7% vs 72.5%,P〈0.01）.There were significant differences on expressions of MMP-9 and E-cadherin in different pathological grade and yes or no lymph node metastasis（P〈0.05 or P〈0.01）.The expression levels of MMP-9 increased and the expression levels of E-cadherin decreased in turn in proportion to the progress of pathological grade（P〈0.05 or P〈0.01）.The expression levels of E-cadhein in deep invasion of esophageal carcinoma were lower than those in shallow invasion of esophageal carcinoma（P〈0.01）.The expression of MMP-9 was negatively correlated with that of E-cadherin（r=-0.269,P〈0.05）.Conclusions The expressions of E-cadherin and MMP-9 could be possibly correlated with the pathogeny,invasion and metastasis of esophageal carcinoma.