基于MRM-MS技术的蛋白质生物标志物研究的前景

Strategies for novel protein biomarker identification through multiple reaction monitoring mass spectrometry

随着蛋白质组学技术的兴起，疾病生物标志物的研究发展迅速，但筛选出的海量候选标志物却极少进入临床应用。近几年，基于MRM—MS技术的方法和策略凭借其低成本、耗时短、高灵敏度、高准确性等优势引起了国内外检验医学界的广泛关注。基于MRM—MS的技术，特别是SISCAPA技术，可能在不远的将来将逐步发展成为蛋白质生物标志物研究的快车道。本文分析了当前生物标志物研究领域的瓶颈，系统阐述了MRM—MS技术的原理和特点，以及近年来该技术在蛋白质生物标志物研究领域的主要应用现状，并展望其发展前景。

With the development of proteomic technologies, large numbers of putative biomarkers have been screened out but few of them are put into the clinical application. Recently, MRM-MS based approaches and strategies have aroused extensive concern in domestic and overseas clinical laboratory community because of their cost-efficiency, time-saving, high analytical sensitivity and accuracy. MRM-MS hased approaches, especially SISCAPA, may gradually become a key technology for the protein biomarker research in the near future. In this review, the ＂ bottleneck＂ existing in the field of protein biomarkers research, and features as well as applications of MRM-MS are discussed and the future trend of research is prospected.