摘要
随着蛋白质组学技术的兴起,疾病生物标志物的研究发展迅速,但筛选出的海量候选标志物却极少进入临床应用。近几年,基于MRM—MS技术的方法和策略凭借其低成本、耗时短、高灵敏度、高准确性等优势引起了国内外检验医学界的广泛关注。基于MRM—MS的技术,特别是SISCAPA技术,可能在不远的将来将逐步发展成为蛋白质生物标志物研究的快车道。本文分析了当前生物标志物研究领域的瓶颈,系统阐述了MRM—MS技术的原理和特点,以及近年来该技术在蛋白质生物标志物研究领域的主要应用现状,并展望其发展前景。
With the development of proteomic technologies, large numbers of putative biomarkers have been screened out but few of them are put into the clinical application. Recently, MRM-MS based approaches and strategies have aroused extensive concern in domestic and overseas clinical laboratory community because of their cost-efficiency, time-saving, high analytical sensitivity and accuracy. MRM-MS hased approaches, especially SISCAPA, may gradually become a key technology for the protein biomarker research in the near future. In this review, the " bottleneck" existing in the field of protein biomarkers research, and features as well as applications of MRM-MS are discussed and the future trend of research is prospected.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2011年第6期484-488,共5页
Chinese Journal of Laboratory Medicine
基金
国家自然科学基金资助项目(81071668)
国家高技术研究发展计划(863计划)资助课题(2006AA022105)