流式荧光杂交法检测咽喉部肿瘤样病变HPV亚型的意义

Significance of human papilloma virus subtypes detected by flexible multi-analyte profiling technology in pharynlarygeal neoplasms

目的探讨咽喉部人乳头状瘤病毒(HPV)感染与良、恶性肿瘤样病变的关系。方法采取25例声带息肉、24例咽喉鳞状上皮乳头状瘤和19例喉鳞状细胞癌临床活检石蜡组织标本,用流式荧光杂交技术检测HPV基因型19种高危亚型和7种低危亚型的表达。结果 HPV感染率在声带息肉组为24%(6/25),咽喉乳头状瘤组为37.5%(9/24),喉鳞癌组为10.5%(2/19)。良性病例HPV亚型感染以HPV6和HPV11亚型为主,鳞癌病例仅为HPV16亚型。结论咽喉部肿瘤样病变的发生与HPV感染密切相关,部分良性病例伴有HPV16亚型感染,可以作为提示恶变倾向的指征。

Objective To explore the relationship between infection of human papilloma virus（HPV） and benign and malignant pharynlarygeal neoplasm diseases.Methods 19 high-risk and 7 low-risk HPV-DNA in paraffin-embedded tissue sections from 25 cases of vocal cords polyps,24 cases of pharynlarygeal papilloma and 19 cases of laryngeal squamous cell carcinoma were detected by flexible multi-analyte profiling technology.Results The positive rate of HPV-DNA was 24%（6/25） in vocal cords polyps,37.5%（9/24） in pharynlarygeal papilloma and 10.5%（2/19） in laryngeal squamous cell carcinoma.The HPV6 and HPV11 DNA were preponderance in pharynlarygeal benign lesions,and HPV16 DNA were preponderance in laryngeal squamous cell carcinoma.Conclusions The infection with HPV is closely associated with the development of pharynlarygeal neoplasm diseases.The part of benign lesions with HPV16 DNA infected can be an indicator that can predict canceration.