NK/T细胞在肺癌组织中的浸润对预后的影响

Infiltration of NK/T cells and its impact on prognosis of lung cancer

目的研究NK/T细胞在肺癌组织中的浸润程度及对预后的影响。方法将CD8作为细胞毒T淋巴细胞(CTL)的标记物,CD56作为自然杀伤细胞(NK)的特异性标记物,应用免疫组化方法检测肿瘤组织中NK/T细胞的分布和浸润情况。结果 100例肺癌组织中,CTL无～轻度浸润者59例;显著浸润者41例,5年生存率分别为22%和40%;NK细胞无～轻度浸润者68例,显著浸润者32例,5年生存率分别为24%和43%;NK/T均显无～轻度浸润者43例,显著浸润者26例,5年生存率分别为33%和54%。经Log-rank检验,NK/T显著浸润组与无～轻度浸润组5年生存率间差异极显著(P<0.01)。结论肺癌组织中NK/T细胞显著浸润组的预后明显好于轻度浸润组,可能与机体细胞免疫有关。

Objective To study the degree of infiltration of NK and T cells in lung cancer and their relation to the survival and prognosis of the patient.Methods CD8 as a marker of cytoxic lymphocyte（CTL） and CD56 as a marker of natural killer（NK） were immunohistochemically detected to study the distribution and infiltration in the lung carcer tissue specimens.Results In 59 of 100 lung cancers,whose CTL infiltration was zero or mild,the five-year survival rate was 22%,while in 41 with marked CTL infiltration,the five-year survival rate was 40%;In 68 of 100 lung neoplams,whose NK cell infiltration was zero or mild,the five-year survival rate was 24%,while in 32 with marked NK cell infiltration,the five-year survival rate was 43%;in 43 of 100 lung neoplams,both the NK and T cells were zero or mild,the five-year survival rate was 33%,while in 26 with marked NK and T cell infiltration,the five-year survival rate was 54%.The five-year survival between the NK and T cells marked and zero or mild infiltration groups were significantly different（P0.01） by Log-rank test.Conclusion The mechanisms which the degree of NK and T infiltration is positively correlated with the prognosis and survival time of lung cancinoma patients may be related to the immune cell function of the body.