125↑I-103↑Pd复合型放射性粒子治疗小鼠肺癌移植瘤的实验观察

Therapeutic effect of 125↑I-183↑pd hybrid radioactive seeds on pulmonary carcinomas in BALB/c-nu mice

目的 制备125↑I-103↑Pd复合型放射性粒子,并探讨其对肺癌的杀伤效果.方法 利用化学镀层方法制备125↑I-103↑Pd放射性粒子.体外培养肺腺癌GLC-82细胞及大细胞癌H460细胞,将125↑I-103↑Pd.125↑I、103↑Pd及空白粒子置于细胞培养皿中照射48 h,观察粒子对肿瘤细胞的杀伤效应.40只BALB/c-nu小鼠随机平分为2组,分别构建GLC-82及H460的移植瘤模型,每种模型荷瘤鼠随机分成_4组,每组5只.将125↑I-103↑pd、125↑I、103↑Pd及空白粒子分别植入裸鼠的移植瘤内,每5天定期测量移植瘤大小及裸鼠体重变化,观察61 d.结果 成功制备125I-103Pd粒子.粒子照射细胞48 h后,125↑I、103↑Pd及125↑I-103↑Pd粒子周围约1个粒子直径范围内的GLC-82细胞明显肿胀、变形,生长抑制;125↑I粒子周围H460细胞牛长无明显改变,而103↑Pd及125↑I-103↑Pd粒子周围约1个粒子直径内H460细胞生长稀少.体内实验小鼠无死亡,H460移植瘤较GLC-82移植瘤生长快,放射性粒子对两种移植瘤均有抑制,抑瘤效果：125↑I-103↑pd粒子〉103↑Pd粒子≈125↑I粒子〉空白粒子;同种放射性粒子对GLC-82移植瘤的抑制效果较H460移植瘤好.结论 125↑I-103↑Pd复合型放射性粒子制备工艺可行,抑瘤效果较好,具有较好的应用前景.

Objective To prepared 125↑I-103↑Pd hybrid radioactive seeds and to explore their therapeutic effect on pulmonary carcinomas.nethods The 125↑I-103↑Pd hybrid radioactive seeds were prepared by a chemical method of step-by-step coat plating.Pulmonary adenocarcinoma of GLC-82 cells and pulmonary large cell carcinoma of H460 cells were cultured in vitro and then were exposed directly to125↑I,103↑pd and125I103pd seeds for 48 hours to observe the killing effects of radiation.GLC-82 and H460 tumor models were established and 20 mice chosen randomly for each model.For each tumor model.there were 4 groups（n=5 each）.Then 125↑I-103↑pd,125↑I,103↑Pd and nonradioactive seeds were implanted into the tumors.Tumor sizes and weights of mice were measured and recorded every 5 days for a 2-month observation.Resuits The125↑I-103↑Pd hybrid radioactive seeds were prepared successfully.After a 48-hour radiation from radioactive seeds, the GLC-82 cells within one particulate around 125↑I,103↑Pd or1 25↑I-103↑Pd seeds were inhibited so as to become swollen and transfiguring.The H460 cells around125↑I seeds showed no obvious abnormality while those within one Darticalate around 103Pd or125↑I-103↑pd seeds were much fewer. No mouse died during the observation period.The radioactive seeds could inhibit the tumors.The radiotherapeutie effects were similar in two tumor modes：125↑I-103↑Pd seeds〉103Pd seeds≈125↑I seeds〉non-radioactive seeds.H460 tumors grew much faster than GLC-82 tumors.Meanwhile the seeds with the same nuclide were much more effective for GLC-82 tumors than for H460 tumors.Conclusion The↑I-l03↑pd hybrid radioactive seeds are clinically applieable due to their effective inhibitions of tumor growth.