摘要
目的 探讨蛇毒型神经生长因子（viper venom nerve growth factor,Vngf）促进脑缺血再灌注损伤后大鼠神经重塑的可能机制.方法 雄性Wistar大鼠90只根据随机数字表法分成5组：Vngf-25 U组、Vngf-50 U组、Vngf-100 U组、缺血再灌注组和假手术组,每组18只.造模后第2、7、14天分批处死大鼠取脑组织提取总RNA,用荧光定量PcR检测候选可塑性相关基因15（cpg-15）Mrna和核转录因子κB（NF-κB）mRNA的表达.结果 随着时间的延长,vNGF各组的cpg-15mRNA和NF-κB mRNA表达逐渐增加（cpg-15和NF-κB的F值分别为：70.43、34.11、31.89和27.47、34.56、31.89,均P＜0.01）;术后同一时问,cpg.15 mRNA和NF.xB mRNA在vNGF各组、缺血再灌注组和假手术组的表达值比较差异有统计学意义（cpg-15和NF-κB的F值分别为：48.18、55.93、78.43和45.92、55.72、50.49,均P＜0.01）,并且cpg-15 mRNA和NF-κB mRNA的表达随着vNGF浓度的升高而增加.结论 脑缺血再灌注损伤后经侧脑室给予vNGF,可以通过正性调节cpg-15和NF-κB转导通路促进神经再生和神经缺损功能恢复.
Objective To investigate the significance and mechanism of intracerebroventricular injection viper venom nerve growth factor （Vngf） in rat neural plasticity after cerebral ischemia reperfusion injury.Methods Ninety Wistar male rats were randomly assigned into Vngf-25 U group （n = 18）, Vngf-50 U group （n = 18）, Vngf-100 U group （n = 18）, ischemia reperfusion group （n = 18） and sham operated group.The expression of candidate plasticity-related gene 15（cpg-15） Mrna and nuclear factor of kappa B （ NF-Κb ） Mrna in rat brain tissues which were collection at 2,7,14 days after surgery were evaluated by the real time PCR.Results The expression of cpg-15 Mrna and NF-Κb Mrna began to increase after surgery（ the F value of cpg-15：70.43, 34.11, 31.89, the F value of NF-Κb： 27.47, 34.56, 31.89,P＜0.01）.At the same time, expression of cpg-15 Mrna and NF-Κb Mrna in the Vngf groups was significantly different from the I/R group and the sham operated group （the F value of cpg-15：48.18, 55.93, 78.43, the F value of NF-Κb： 45.92, 55.72, 50.49, P ＜0.01）.The more Vngf were injected, the more cpg-15 Mrna and NF-Κb Mrna were expressed in Vngf groups.Conclusions The Vngf could accelerate neural plasticity and restore neurofunctional defect through up-regulated the expression of cpg-15 and NF-Κb.
出处
《中华神经科杂志》
CAS
CSCD
北大核心
2011年第6期389-392,共4页
Chinese Journal of Neurology
