长寿花嫩叶片的组织培养研究

Tissue Culture from Seedling Leaf of Kalanchoe blossfeldiana

以长寿花嫩叶片为外植体,进行了不同灭菌时间对长寿花嫩叶片再生能力的影响实验,不同培养基对长寿花嫩叶片愈伤组织诱导及不定芽生根的影响实验。实验结果表明,长寿花嫩叶片的最佳消毒方法为,75%酒精灭菌30 s,无菌水冲洗4次,再用0.1%升汞消毒12 m in,最后无菌水冲洗7次;长寿花嫩叶片的最佳分化培养基为MS+6-BA3.5 mg/L+NAA0.2 mg/L;长寿花不定芽在1/2MS+IBA 0.15 mg/L+6-BA 0.15 mg/L上生根效果最好。通过实验获得了长寿花嫩叶片的组培技术,为大规模生产长寿花提供了相关技术支撑。

Using explant from seedling leaf of Kalanchoe blossfeldiana, the regeneration ahility of the leaf of Kal- anchoe blossfeldiana was tested under different duration of sterilization, and effect of induction for the formation of callus and adventive root in different types of culture. It showed that best way of sterilization was sterilizing the tissues in 75% ethanol for 30 seconds, washing 4 times with disinfection water, then in 0. 1% mercury bichloride for 12 minutes and washing with disinfection water for 7 times; best culture for the differentiation of seedling leaf was MS ＋ 6 - BA3. 5 mg/L ＋ NAAO. 2 mg/L ; and the formation of callus and adventive root were better in the culture of 1/2MS ＋IBA 0. 15mg/L＋6-BA 0. 15mg/L.